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Agrisera
EF-G1/elongation factor G1 Polyclonal Antibody
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FIGURE: 1 / 1
EF-G1/elongation factor G1 Antibody (AS13-2652) in WB
Left panel: from 0.15 to 5.17 µg of total protein from Synechocystis PCC 6803 extracted with SDS-sample buffer and recombinant EF-G 1 (right panel) were separated on 10% SDS-PAGE and blotted 1 h to PVDF. Blots were blocked with 5% milk powder in TBS-T for 30 min. at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:4 000 for 1 h at RT with agitation. The ant... View More
Product Details
AS13-2652
Product Specifications
Species Reactivity
Bacteria
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant EF-G1 protein from Synechocystis.
Conjugate
Form
Lyophilized
Storage buffer
whole serum
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Streptococcus pneumoniae, Synechocystis sp. PCC6803
Target Information
EFG1 encodes a sequence-specific DNA-binding transcription factor that functions primarily in the nucleus to coordinate transcriptional programs underlying morphogenesis and host-associated traits in the opportunistic fungal pathogen Candida albicans. The Efg1 protein is an APSES-family regulator with a central conserved DNA-binding region that includes a basic helix-loop-helix (bHLH)-like motif and surrounding APSES sequences required for DNA binding and normal morphogenetic outputs; additional low-complexity regions, including polyglutamine-rich segments, contribute to protein stability and context-dependent regulatory activity. Functionally, Efg1 is essential for hyphal development under many inducing conditions and directly binds E-box-type DNA elements, acting as both activator and repressor to modulate expression of morphology- and cell-surface/cell-wall-associated genes (including hypha-specific and yeast-associated transcripts), thereby influencing filamentation, cell wall dynamics, and broader virulence-linked behaviors such as biofilm-associated phenotypes and interactions with host-relevant substrates.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: COXPD1; EFG; EFGM; EGF1; GFM; hEFG1; mtEF-G1
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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