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Agrisera
EF1A/Elongation factor 1-alpha/EF-1-alpha Polyclonal Antibody
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FIGURE: 1 / 1
EF1A/Elongation factor 1-alpha/EF-1-alpha Antibody (AS11-1633) in WB
10 µg total protein extracted from 15 day old Arabidopsis thaliana seedlings that were either kept at 22°C (3) or heat treated at 38°C for 2 hours prior to protein extraction (4). As positive control 10 ng of recombinant At EF 1 A (2) were separated side by side with the plant samples on 10-15% gradient SDS-PAGE and blotted to nitrocellulose (Bio-Rad). Blots were blocked following transfer with 5% low fat m... View More
Product Details
AS11-1633
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
Full-length, recombinant EF1A of Arabidopsis thaliana.
Conjugate
Form
Lyophilized
Storage buffer
whole serum
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 25 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana, Oryza sativa, Triticum aestivum
Target Information
EF1A1, also called elongation factor 1 alpha 1, is a highly abundant cytosolic GTP binding protein that delivers aminoacyl tRNAs to the ribosome during the elongation phase of protein synthesis in plants. It cycles between GTP and GDP bound states, interacting with EF1B for nucleotide exchange and ensuring translational accuracy and efficiency. Beyond translation, EF1A1 binds actin and microtubules, contributing to cytoskeleton organization, vesicle trafficking, and responses to stress. Its expression is strong in growing tissues and can change with environmental conditions, pathogen challenge, and developmental cues. EF1A1 is often used as a reference gene for normalization in expression studies due to its stable baseline, though stability can vary with treatments, so validation is recommended.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CCS-3; CCS3; EE1A1; EEF-1; EEF1A; eEF1A-1; EF-Tu; EF1A; EF1A1; EF1ALPHA1; GRAF-1EF; LENG7; PTI1
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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