Invitrogen

EGFR Monoclonal Antibody (111.6)

Advanced Verification

This Antibody was verified by Independent antibody to ensure that the antibody binds to the antigen stated.

3 Published Figures

62 References

View all (257) EGFR antibodies

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EGFR Antibody (MA5-13269) in WB

Western blot was performed using Anti-EGFR Monoclonal Antibody (111.6) (Product # MA5-13269) and a 130 kDa band corresponding to EGFR was observed across tissues and cell lines tested. Membrane enriched extracts (30 µg lysate) of Mouse Placenta (Lane 1), Mouse Brain (Lane 2), Rat Brain (Lane 3), Mouse Liver (Lane 4), Rat Liver (Lane 5), A-431 (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry... View More

Product Image Gallery

EGFR Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma stained with Epidermal Growth Factor Receptor antibody0 using peroxidase-conjugate and AEC chromogen. Note membrane staining of tumor cells.

EGFR Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of EGFR was performed using 90% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with EGFR Mouse monoclonal antibody (Product # MA5-13269) at 5 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membrane localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

EGFR Antibody in Immunoprecipitation (IP) — Immunoprecipitation of Epidermal Growth Factor Receptor using Epidermal Growth Factor Receptor Monoclonal Antibody (Product # MA5-13269) on Native Human A431 Cells.

EGFR Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. A higher level of expression of EGFR was observed in A-431 compared to HeLa and A549 using EGFR Mouse Monoclonal Antibody (Product # MA5-13269) in Western Blot.(DOI: 10.1038/cr.2009.53). {RE}

Product Details

MA5-13269

Applications

Tested Dilution

Publications

Western Blot (WB)

2-4 µg/mL

View 7 publications 7 publications

Immunohistochemistry (IHC)

View 32 publications 32 publications

Immunohistochemistry (Paraffin) (IHC (P))

2-4 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

5 µg/mL

View 12 publications 12 publications

Flow Cytometry (Flow)

View 4 publications 4 publications

ELISA (ELISA)

View 1 publication 1 publication

Immunoprecipitation (IP)

2 µg/mL

View 3 publications 3 publications

Neutralization (Neu)

View 1 publication 1 publication

Gel Shift (GS)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Bat, Dog, Human, Mouse, Non-human primate, Sheep

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

111.6

Immunogen

Extracellular domain of human recombinant EGFR protein

Conjugate

Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_10978855

Product Specific Information

MA5-13269 targets Epidermal Growth Factor Receptor in IHC (P), IP, and WB applications and shows reactivity with Human samples.

The MA5-13269 immunogen is extracellular domain of human recombinant EGFR protein.

Target Information

EGFR, epidermal growth factor receptor, is a receptor tyrosine kinases that signals in response to various growth factors. Overexpression has been linked to numerous types of cancer and EGFR is the target of both biological and small molecular therapeutics. EGFR is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Golgi Acidification by NHE7 Regulates Cytosolic pH Homeostasis in Pancreatic Cancer Cells.

Cancer discovery

Galenkamp KMO,Sosicka P,Jung M,Recouvreux MV,Zhang Y,Moldenhauer MR,Brandi G,Freeze HH,Commisso C

MA5-13269 was used in Immunocytochemistry to identify Golgi acidification as a mechanism to control pHi and point to the regulation of pHi as a possible therapeutic vulnerability in pancreatic ductal adenocarcinoma (PDAC).

Mon Jun 01 00:00:00 EDT 2020

Exosomes derived from oviduct cells mediate the EGFR/MAPK signaling pathway in cumulus cells.

Journal of cellular physiology

Lee SH,Oh HJ,Kim MJ,Lee BC

MA5-13269 was used in Immunocytochemistry-immunofluorescence to provide the first evidence that OC-Exo effectively enhance the physiological condition of cumulus cells exposed to GW4869 or Gefitinib via the EGFR/MAPK signaling pathway.

Sat Feb 01 00:00:00 EST 2020

Exosomes derived from oviduct cells mediate the EGFR/MAPK signaling pathway in cumulus cells.

Journal of cellular physiology

Lee SH,Oh HJ,Kim MJ,Lee BC

Sat Feb 01 00:00:00 EST 2020

β-Catenin/CBP inhibition alters epidermal growth factor receptor fucosylation status in oral squamous cell carcinoma.

Molecular omics

Chandler KB,Alamoud KA,Stahl VL,Nguyen BC,Kartha VK,Bais MV,Nomoto K,Owa T,Monti S,Kukuruzinska MA,Costello CE

MA5-13269 was used in Immunoprecipitation to demonstrate the role of the β-catenin/CBP axis in epidermal growth factor receptor oncogenic activity in oral squamous cell carcinoma.

Mon Jun 01 00:00:00 EDT 2020

Interaction of the EGFR signaling pathway with porcine cumulus oocyte complexes and oviduct cells in a coculture system.

Journal of cellular physiology

Lee SH,Oh HJ,Kim MJ,Setyawan EMN,Lee BC

Published figure using EGFR monoclonal antibody (Product # MA5-13269) in Immunofluorescence

Mon Apr 01 00:00:00 EDT 2019

A Chlamydia pneumoniae adhesin induces phosphatidylserine exposure on host cells.

Nature communications

Galle JN,Fechtner T,Eierhoff T,Römer W,Hegemann JH

Published figure using EGFR monoclonal antibody (Product # MA5-13269) in Immunocytochemistry

Fri Oct 11 00:00:00 EDT 2019

Multiplexed Molecular Imaging of Biomarker-Targeted SERS Nanoparticles on Fresh Tissue Specimens with Channel-Compressed Spectrometry.

PloS one

Kang S,Wang Y,Reder NP,Liu JT

MA513269 was used in immunohistochemistry to perform Raman-encoded molecular imaging with five nanoparticle flavors

Wed Nov 20 00:00:00 EST 2019

Quantitative molecular phenotyping with topically applied SERS nanoparticles for intraoperative guidance of breast cancer lumpectomy.

Scientific reports

Wang Y,Kang S,Khan A,Ruttner G,Leigh SY,Murray M,Abeytunge S,Peterson G,Rajadhyaksha M,Dintzis S,Javid S,Liu JT

MA5-13269 was used in flow cytometry to learn about intraoperative guidance of breast cancer lumpectomy by quantitative molecular phenotyping with topically applied SERS nanoparticles

Tue Feb 16 00:00:00 EST 2016

Missense Mutations in Exons 18-24 of EGFR in Hepatocellular Carcinoma Tissues.

BioMed research international

Panvichian R,Tantiwetrueangdet A,Sornmayura P,Leelaudomlipi S

MA5-13269 was used in immunohistochemistry - paraffin section to characterize hepatocellular carcinoma tissues and missense mutations in exons 18-24 of EGFR

Tue Jul 19 00:00:00 EDT 2016

An early endosome regulator, Rab5b, is an LRRK2 kinase substrate.

Journal of biochemistry

Yun HJ,Kim H,Ga I,Oh H,Ho DH,Kim J,Seo H,Son I,Seol W

MA5-13269 was used in western blot to elucidate the relationship between LRRK2 and Rab5b

Mon Jun 01 00:00:00 EDT 2015

Quantification of the binding potential of cell-surface receptors in fresh excised specimens via dual-probe modeling of SERS nanoparticles.

Scientific reports

Sinha L,Wang Y,Yang C,Khan A,Brankov JG,Liu JT,Tichauer KM

MA5-13269 was used in ELISA to use of dual-probe modeling of SERS nanoparticles to quantify binding potential of cell-surface receptors in fresh excised specimens

Thu Feb 26 00:00:00 EST 2015

Single cell stochastic regulation of pilus phase variation by an attenuation-like mechanism.

PLoS pathogens

Danne C,Dubrac S,Trieu-Cuot P,Dramsi S

MA5-13269 was used in immunohistochemistry to study the molecular mechanisms underlying pilus phase variation

Wed Jan 01 00:00:00 EST 2014

EGFR, HER2 and HER3 dimerization patterns guide targeted inhibition in two histotypes of esophageal cancer.

International journal of cancer

Fichter CD,Timme S,Braun JA,Gudernatsch V,Schöpflin A,Bogatyreva L,Geddert H,Faller G,Klimstra D,Tang L,Hauschke D,Werner M,Lassmann S

MA5-13269 was used in immunocytochemistry and immunohistochemistry to study the value of the expression and dimerization patterns of EGFR, HER2 and HER3 in esophageal cancer histotypes for guiding targeted anti-EGFR and -HER2 therapy

Wed Oct 01 00:00:00 EDT 2014

Integrins and their ligands are expressed in non-small cell lung cancer but not correlated with parameters of disease progression.

Virchows Archiv : an international journal of pathology

Böger C,Kalthoff H,Goodman SL,Behrens HM,Röcken C

MA5-13269 was used in immunohistochemistry to study the progression of non-small cell lung cancer and the lack of correlation with the expression of integrins and their ligands

Wed Jan 01 00:00:00 EST 2014

Role of EGFR as a prognostic factor for survival in head and neck cancer: a meta-analysis.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Keren S,Shoude Z,Lu Z,Beibei Y

MA5-13269 was used in immunohistochemistry to study the prognostic value of EGFR in head and neck cancers

Sat Mar 01 00:00:00 EST 2014

EGFR, HER-2 and KRAS in canine gastric epithelial tumors: a potential human model?

PloS one

Terragni R,Casadei Gardini A,Sabattini S,Bettini G,Amadori D,Talamonti C,Vignoli M,Capelli L,Saunders JH,Ricci M,Ricci M,Ulivi P,Ulivi P

MA5-13269 was used in immunohistochemistry to examine canine gastric epithelial tumors for the expression of EGFR, HER-2 and KRAS in order to determine whether dog is a suitable model of the corresponding human cancer

Mon Dec 29 00:00:00 EST 2014

No-cost manual method for preparation of tissue microarrays having high quality comparable to semiautomated methods.

Applied immunohistochemistry & molecular morphology : AIMM

Foda AA

MA5-13269 was used in immunohistochemistry to optimize a manual method for the production of cheap, high quality tissue microarrays

Wed May 01 00:00:00 EDT 2013

Receptor tyrosine kinase inhibitor profiling using bead-based multiplex sandwich immunoassays.

Methods in molecular biology (Clifton, N.J.)

Pötz O,Schneiderhan-Marra N,Henzler T,Herget T,Joos TO

MA5-13269 was used in blocking or activating experiment to develop a bead-based sandwich immunoassay for the profiling of receptor tyrosine kinase inhibitors

Thu Jan 26 00:00:00 EST 2012

An aCGH classifier derived from BRCA1-mutated breast cancer and benefit of high-dose platinum-based chemotherapy in HER2-negative breast cancer patients.

Annals of oncology : official journal of the European Society for Medical Oncology

Vollebergh MA,Lips EH,Nederlof PM,Wessels LFA,Schmidt MK,van Beers EH,Cornelissen S,Holtkamp M,Froklage FE,de Vries EGE,Schrama JG,Wesseling J,van de Vijver MJ,van Tinteren H,de Bruin M,Hauptmann M,Rodenhuis S,Linn SC

MA5-13269 was used in immunohistochemistry to investigate an aCGH classifier for BRCA1 loss in breast cancer

Fri Jul 01 00:00:00 EDT 2011

Epidermal growth factor receptor as a biomarker for cervical cancer.

Annals of oncology : official journal of the European Society for Medical Oncology

Soonthornthum T,Arias-Pulido H,Joste N,Lomo L,Muller C,Rutledge T,Verschraegen C

MA5-13269 was used in immunohistochemistry to investigate the expression of epidermal growth factor receptor in cervical cancer

Sat Oct 01 00:00:00 EDT 2011

Transdifferentiation of glioblastoma cells into vascular endothelial cells.

Proceedings of the National Academy of Sciences of the United States of America

Soda Y,Marumoto T,Friedmann-Morvinski D,Soda M,Liu F,Michiue H,Pastorino S,Yang M,Hoffman RM,Kesari S,Verma IM

MA5-13269 was used in immunohistochemistry to study the transdifferentiation of glioblastoma cells into vascular endothelial cells and the mechanism of resistance to anti-VEGF therapy.

Tue Mar 15 00:00:00 EDT 2011

Expression of epidermal growth factor receptor and vascular endothelial growth factor in vaginal squamous cell cancer.

American journal of obstetrics and gynecology

Brunner A,Grimm C,Polterauer S,Hefler L,Stani J,Dudek G,Horvat R

MA5-13269 was used in immunohistochemistry to investigate VEGF and EGFR in vaginal squamous cell cancer

Tue Feb 01 00:00:00 EST 2011

Identification of different subtypes of breast cancer using tissue microarray.

Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie

Munirah MA,Siti-Aishah MA,Reena MZ,Sharifah NA,Rohaizak M,Norlia A,Rafie MK,Asmiati A,Hisham A,Fuad I,Shahrun NS,Das S

MA5-13269 was used in immunohistochemistry to evaluate tissue microarray for classification of human breast cancer

Wed Sep 28 00:00:00 EDT 2011

Comparison of four antibodies for immunohistochemical evaluation of epidermal growth factor receptor expression in non-small cell lung cancer.

Lung cancer (Amsterdam, Netherlands)

Mathieu A,Weynand B,Verbeken E,Da Silva S,Decaestecker C,Salmon I,Demetter P

MA5-13269 was used in immunohistochemistry to investigate the performance of four different anti-EGFR antibodies in immunohistochemical study of non-small cell lung cancer

Thu Jul 01 00:00:00 EDT 2010

Triple-negative breast cancer: immunohistochemical correlation with basaloid markers and prognostic value of survivin.

Medical oncology (Northwood, London, England)

Dogu GG,Ozkan M,Ozturk F,Dikilitas M,Er O,Ozturk A

MA5-13269 was used in immunohistochemistry to study the prognostic value of survivin in patients with basaloid triplet-negative breast cancer

Mon Mar 01 00:00:00 EST 2010

MAP Kinase activation by receptor tyrosine kinases: in control of cell migration.

Methods in molecular biology (Clifton, N.J.)

Tarcic G,Yarden Y

MA5-13269 was used in flow cytometry to review high throughput methods for profiling the activation of signalling molecules.

Tue Dec 14 00:00:00 EST 2010

Ultraviolet irradiation-induces epidermal growth factor receptor (EGFR) nuclear translocation in human keratinocytes.

Journal of cellular biochemistry

Xu Y,Shao Y,Zhou J,Voorhees JJ,Fisher GJ

MA5-13269 was used in immunocytochemistry to investigate the induction of EGFR nuclear translocation by ultraviolet irradiation in human Keratinocytes

Sat Aug 01 00:00:00 EDT 2009

Prostaglandin E1 and misoprostol increase epidermal growth factor production in 3D-cultured human annulus cells.

The spine journal : official journal of the North American Spine Society

Gruber HE,Hoelscher G,Loeffler B,Chow Y,Ingram JA,Halligan W,Hanley EN

MA5-13269 was used in immunocytochemistry to investigate the influence of PGE1 and misoprostol on epidermal growth factor expression in 3D-cultured human annulus cells

Tue Sep 01 00:00:00 EDT 2009

EGFR and ADAMs cooperate to regulate shedding and endocytic trafficking of the desmosomal cadherin desmoglein 2.

Molecular biology of the cell

Klessner JL,Desai BV,Amargo EV,Getsios S,Green KJ

MA5-13269 was used in western blot to examine the role of EGFR and ADAMs in the endocytic trafficking of the desmosomal cadherin desmoglein 2

Thu Jan 01 00:00:00 EST 2009

Structure-function analysis of nucleolin and ErbB receptors interactions.

PloS one

Farin K,Di Segni A,Mor A,Pinkas-Kramarski R

MA5-13269 was used in western blot to investigate the interaction between nucleolin and ErbB receptors

Fri Jul 03 00:00:00 EDT 2009

Engineering of PDMS surfaces for use in microsystems for capture and isolation of complex and biomedically important proteins: epidermal growth factor receptor as a model system.

Lab on a chip

Lowe AM,Ozer BH,Wiepz GJ,Bertics PJ,Abbott NL

MA5-13269 was used in immunoprecipitation to develop a novel system for capture and isolation of biomedically important proteins

Fri Aug 01 00:00:00 EDT 2008

The role of heparin-binding EGF-like growth factor and amphiregulin in the epidermal proliferation of psoriasis in cooperation with TNFalpha.

Archives of dermatological research

Yoshida A,Kanno H,Watabe D,Akasaka T,Sawai T

MA5-13269 was used in immunohistochemistry to examine the role of growth factors in epidermal hyperplasia of psoriasis

Tue Jan 01 00:00:00 EST 2008

TGFalpha and EGFR in ovine preimplantation embryos and effects on development.

Animal reproduction science

Zhou P,Liu DJ,Cang M,Ma YZ,Yang DS,Li HJ,Wang LM,Bou S,Feng HL

MA5-13269 was used in immunohistochemistry to investigate the expression of transforming growth factor alpha and its receptor in ovine preimplantation embryos

Mon Mar 03 00:00:00 EST 2008

Sex steroid and epidermal growth factor profile of giant meningiomas associated with pregnancy.

Surgical neurology

Hatiboglu MA,Cosar M,Iplikcioglu AC,Ozcan D

MA5-13269 was used in immunohistochemistry to investigate the distribution of specific proteins in giant meningiomas associated with pregnancy

Tue Apr 01 00:00:00 EDT 2008

Gene expression profiling and histopathological characterization of triple-negative/basal-like breast carcinomas.

Breast cancer research : BCR

Kreike B,van Kouwenhove M,Horlings H,Weigelt B,Peterse H,Bartelink H,van de Vijver MJ

MA5-13269 was used in immunohistochemistry to study triple negative breast carcinoma histopathology and gene expression patterns in order to define clinically significant sub-groups

Tue Mar 11 00:00:00 EDT 2008

Epidermal growth factor ligand/receptor loop and downstream signaling activation pattern in completely resected nonsmall cell lung cancer.

Cancer

Volante M,Saviozzi S,Rapa I,Ceppi P,Cappia S,Calogero R,Novello S,Borasio P,Papotti M,Scagliotti GV

MA5-13269 was used in immunohistochemistry to study EGF receptor pathway activation in completely resected non-small cell lung cancer

Sat Sep 15 00:00:00 EDT 2007

Basaloid adenocarcinoma. A new variant of pulmonary adenocarcinoma.

Virchows Archiv : an international journal of pathology

Marci V,Volante M,Cappia S,Righi L,Novello C,Scagliotti GV,Brambilla E,Papotti M

MA5-13269 was used in immunohistochemistry to report a clinical case of basaloid adenocarcinoma

Sat Sep 01 00:00:00 EDT 2007

Co-expression of EGF receptor, TGFalpha and S6 kinase is significantly associated with colorectal carcinomas with distant metastases at diagnosis.

Virchows Archiv : an international journal of pathology

Tampellini M,Longo M,Cappia S,Bacillo E,Alabiso I,Volante M,Dogliotti L,Papotti M

MA5-13269 was used in immunohistochemistry to study the expression EGF receptor, TGF alpha and S6 kinase as predictors of metastasis in colorectal cancer

Thu Mar 01 00:00:00 EST 2007

Dual-mode reflectance and fluorescence near-video-rate confocal microscope for architectural, morphological and molecular imaging of tissue.

Journal of microscopy

Carlson AL,Coghlan LG,Gillenwater AM,Richards-Kortum RR

MA5-13269 was used in immunocytochemistry to develop a dual-mode reflectance and fluorescence confocal microscope for imaging tissue samples with high resolution

Mon Oct 01 00:00:00 EDT 2007

Downregulation of EGF receptor signaling in pancreatic islets causes diabetes due to impaired postnatal beta-cell growth.

Diabetes

Miettinen PJ,Ustinov J,Ormio P,Gao R,Palgi J,Hakonen E,Juntti-Berggren L,Berggren PO,Otonkoski T

MA5-13269 was used in immunohistochemistry and western blot to study the role of EGF receptor signaling in postnatal beta-cell growth in pancreatic islets

Fri Dec 01 00:00:00 EST 2006

Molecular subtypes of breast cancer and amplification of topoisomerase II alpha: predictive role in dose intensive adjuvant chemotherapy.

British journal of cancer

Hannemann J,Kristel P,van Tinteren H,Bontenbal M,van Hoesel QG,Smit WM,Nooij MA,Voest EE,van der Wall E,Hupperets P,de Vries EG,Rodenhuis S,van de Vijver MJ

MA5-13269 was used in immunohistochemistry to study molecular subtypes of breast cancer and amplification of topoisomerase II alpha

Mon Nov 20 00:00:00 EST 2006

Real-time detection of epidermal growth factor receptor expression in fresh oral cavity biopsies using a molecular-specific contrast agent.

International journal of cancer

Hsu ER,Gillenwater AM,Hasan MQ,Williams MD,El-Naggar AK,Richards-Kortum RR

MA5-13269 was used in immunohistochemistry to develop a novel targeted fluorescent contrast agent for EGFR expression in oral neoplasia

Thu Jun 15 00:00:00 EDT 2006

Does the EGFR and VEGF expression predict the prognosis in colon cancer?

European surgical research. Europaische chirurgische Forschung. Recherches chirurgicales europeennes

Doger FK,Meteoglu I,Tuncyurek P,Okyay P,Cevikel H

MA5-13269 was used in immunohistochemistry to examine the prognostic value of EGFR and VEGF expression for colon cancer

Fri Dec 22 00:00:00 EST 2006

Using liquid crystals to report membrane proteins captured by affinity microcontact printing from cell lysates and membrane extracts.

Journal of the American Chemical Society

Jang CH,Tingey ML,Korpi NL,Wiepz GJ,Schiller JH,Bertics PJ,Abbott NL

MA5-13269 was used in immunoprecipitation to develop a liquid crystal surface for analysising membrane proteins in cell lysates and membrane extracts

Wed Jun 29 00:00:00 EDT 2005

Epidermal growth factor receptor, protein kinase B/Akt, and glioma response to erlotinib.

Journal of the National Cancer Institute

Haas-Kogan DA,Prados MD,Tihan T,Eberhard DA,Jelluma N,Arvold ND,Baumber R,Lamborn KR,Kapadia A,Malec M,Berger MS,Stokoe D

MA5-13269 was used in immunohistochemistry to investigate the effect of erlotinib on the expression of epidermal growth factor receptor and other signaling components

Wed Jun 15 00:00:00 EDT 2005

Gene expression and immunolocalization of heparin-binding epidermal growth factor-like growth factor and human epidermal growth factor receptors in human corpus luteum.

Human reproduction (Oxford, England)

Akayama Y,Takekida S,Ohara N,Tateiwa H,Chen W,Nakabayashi K,Maruo T

MA5-13269 was used in immunohistochemistry to study the expression and immunolocalization of HB-EGF-like growth factor and EGFR in human corpus luteum

Sat Oct 01 00:00:00 EDT 2005

Fluorescent nanocrystals for use in early cervical cancer detection.

Gynecologic oncology

Nida DL,Rahman MS,Carlson KD,Richards-Kortum R,Follen M

MA5-13269 was used in immunocytochemistry to evaluate quantum dots for early cervical cancer detection

Thu Dec 01 00:00:00 EST 2005

Cyclooxygenase-2 (COX-2) levels before and after chemotherapy: a study in rectal cancer.

American journal of clinical oncology

Watwe V,Javle M,Lawrence D,Groth J,Iyer R,El-Hajjar D,Geradts J

MA5-13269 was used in immunohistochemistry to study the immunohistochemical expression of Cox-2 before and after chemoradiation therapy of rectal cancer

Thu Dec 01 00:00:00 EST 2005

Sulindac metabolites inhibit epidermal growth factor receptor activation and expression.

Journal of carcinogenesis

Pangburn HA,Kraus H,Ahnen DJ,Rice PL

MA5-13269 was used in western blot to test if sulindac metabolites block activation or expression of the epidermal growth factor receptor

Fri Sep 02 00:00:00 EDT 2005

Activity of a specific inhibitor, gefitinib (Iressa, ZD1839), of epidermal growth factor receptor in refractory non-small-cell lung cancer.

Annals of oncology : official journal of the European Society for Medical Oncology

Santoro A,Cavina R,Latteri F,Zucali PA,Ginanni V,Campagnoli E,Ferrari B,Morenghi E,Pedicini V,Roncalli M,Alloisio M,Ravasi G,Soto Parra HJ

MA5-13269 was used in immunocytochemistry to study effect of the EGFR-selective inhibitor gefitinib in refractory non-small-cell lung cancer

Thu Jan 01 00:00:00 EST 2004

Racial disparity of epidermal growth factor receptor expression in prostate cancer.

Journal of clinical oncology : official journal of the American Society of Clinical Oncology

Shuch B,Mikhail M,Satagopan J,Lee P,Yee H,Chang C,Cordon-Cardo C,Taneja SS,Osman I

MA5-13269 was used in immunohistochemistry to study racial differences in EGFR expression in prostate cancer

Wed Dec 01 00:00:00 EST 2004

In vitro chemoresistance and biomarker profiles are unique for histologic subtypes of epithelial ovarian cancer.

Gynecologic oncology

Cloven NG,Kyshtoobayeva A,Burger RA,Yu IR,Fruehauf JP

MA5-13269 was used in immunohistochemistry to study whether there is a relationship between histologic subtype of epithelial ovarian cancer and chemoresistance

Thu Jan 01 00:00:00 EST 2004

Inability of immunohistochemistry to predict clinical outcomes of endometrial cancer patients.

International journal of gynecological cancer : official journal of the International Gynecological Cancer Society

Gossett DR,Alo P,Bristow RE,Galati M,Kyshtoobayeva A,Fruehauf J,Montz FJ

MA5-13269 was used in immunohistochemistry to study the inability of immunohistochemistry to predict clinical outcome in endometrial cancer

Thu May 27 00:00:00 EDT 2004

Characterization of a transplantable hormone-responsive human prostatic cancer xenograft TEN12 and its androgen-resistant sublines.

The Prostate

Harper ME,Goddard L,Smith C,Nicholson RI

MA5-13269 was used in immunocytochemistry to investigate the effectiveness of Xenograft TEN12 and its sublines on the progression of prostatic cancer

Thu Jan 01 00:00:00 EST 2004

Domain-level antibody epitope mapping through yeast surface display of epidermal growth factor receptor fragments.

Journal of immunological methods

Cochran JR,Kim YS,Olsen MJ,Bhandari R,Wittrup KD

MA5-13269 was used in flow cytometry to study the use of yeast surface display for identifying stable folded protein domains and characterizing antibody binding epitopes

Thu Apr 01 00:00:00 EST 2004

Geldanamycin-associated inhibition of intracellular trafficking is attributed to a co-purified activity.

The Journal of biological chemistry

Barzilay E,Ben-Califa N,Supino-Rosin L,Kashman Y,Hirschberg K,Elazar Z,Neumann D

MA5-13269 was used in western blot to study a co-purifying contaminant of geldanamycin preparations as the component inhibiting intracellular trafficking

Fri Feb 20 00:00:00 EST 2004

A pharmacodynamic study of the epidermal growth factor receptor tyrosine kinase inhibitor ZD1839 in metastatic colorectal cancer patients.

Clinical cancer research : an official journal of the American Association for Cancer Research

Daneshmand M,Parolin DA,Hirte HW,Major P,Goss G,Stewart D,Batist G,Miller WH,Matthews S,Seymour L,Lorimer IA

MA5-13269 was used in immunohistochemistry to study the pharmacodynamics of an EGFR tyrosine kinase inhibitor in metastatic colorectal cancer patients

Tue Jul 01 00:00:00 EDT 2003

Profiling receptor tyrosine kinase activation by using Ab microarrays.

Proceedings of the National Academy of Sciences of the United States of America

Nielsen UB,Cardone MH,Sinskey AJ,MacBeath G,Sorger PK

MA5-13269 was used in flow cytometry to study the receptor tyrosine kinase activation by through antibody microarrays

Tue Aug 05 00:00:00 EDT 2003

Validation of tissue array technology in head and neck squamous cell carcinoma.

Head & neck

Chen B,van den Brekel MW,Buschers W,Balm AJ,van Velthuysen ML

MA5-13269 was used in immunohistochemistry to evaluate the use of tissue array technology to analyse archived head and neck cancer tissue samples

Sat Nov 01 00:00:00 EST 2003

Broadly distributed chemical reactivity of natural antibodies expressed in coordination with specific antigen binding activity.

The Journal of biological chemistry

Planque S,Taguchi H,Burr G,Bhatia G,Karle S,Zhou YX,Nishiyama Y,Paul S

MA5-13269 was used in EMSA to investigate the nucleophilic reactivity of natural antibodies

Fri May 30 00:00:00 EDT 2003

Retroperitoneal cystic papillary carcinoma of probable nephrogenic origin.

Scandinavian journal of urology and nephrology

Praetorius L,Krogh J,Horn T

MA5-13269 was used in immunocytochemistry to report on a case of retroperitoneal cystic papillary carcinoma of likely nephrogenic origin

Sun Apr 01 00:00:00 EST 2001

A sandwich type acridinium-linked immunosorbent assay (ALISA) detects soluble ErbB1 (sErbB1) in normal human sera.

Journal of immunological methods

Baron AT,Lafky JM,Connolly DC,Peoples J,O'Kane DJ,Suman VJ,Boardman CH,Podratz KC,Maihle NJ

MA5-13269 was used in western blot to develop an acridium-linked immunosorbent assay for measuring soluble ErbB1 in human serum

Thu Oct 01 00:00:00 EDT 1998

Bioinformatics

Protein Aliases: 2.7.10.1; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; avian erythroblastic leukemia viral (v-erbB) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10.1; EGFR-related peptide; Epidermal growth factor receptor; Epidermal growth factor receptor formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); epidermal growth factor receptor, formerly avian erythroblastic leukemia viral (v-erbB) oncogene homolog (Erbb1); erb-b2 receptor tyrosine kinase 1; kinase EGFR; Oncogene ERBB; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; waved 2

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Gene Aliases: 9030024J15Rik; AI552599; EGFR; ERBB; ErbB-1; ERBB1; Errb1; Errp; HER1; mENA; NISBD2; PIG61; wa-2; wa2; Wa5

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UniProt ID: (Human) P00533, (Mouse) Q01279

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Entrez Gene ID: (Human) 1956, (Mouse) 13649, (Rat) 24329

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Molecular Function: transmembrane signal receptor

Function(s)

Process(es)

chromatin binding double-stranded DNA binding MAP kinase kinase kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity receptor signaling protein tyrosine kinase activity transmembrane signaling receptor activity epidermal growth factor-activated receptor activity Ras guanyl-nucleotide exchange factor activity protein binding ATP binding enzyme binding protein phosphatase binding nitric-oxide synthase regulator activity ubiquitin protein ligase binding identical protein binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity protein heterodimerization activity actin filament binding cadherin binding involved in cell-cell adhesion nucleotide binding glycoprotein binding protein kinase activity signal transducer activity receptor binding integrin binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein kinase binding epidermal growth factor binding

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EGFR Monoclonal Antibody (111.6)

EGFR Antibody (MA5-13269) in WB

Product Details

MA5-13269

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Immunoprecipitation (IP)

Neutralization (Neu)

Gel Shift (GS)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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