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          • Primary Antibodies ›
          • eIF2A Antibodies

          Rockland

          EIF2A Polyclonal Antibody

          View all (13) eIF2A antibodies

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          Cite EIF2A Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (4)
          EIF2A Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          EIF2A Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 4

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          EIF2A Antibody (600-101-ML7) in ICC/IF

          Immunofluorescence of Goat Anti-EIF2A Antibody. Cells: HeLa Cells. Fixative: 4% PFA. Permeabilization: 0.3% Triton X-100. Primary Antibody: Anti-EIF2A at 5µg/mL overnight at 2-8°C. Secondary Antibody: Donkey Anti-Goat IgG DyLight™488 (p/n 605-741-125) at 5µg/mL for 1hr at RT. Nuclear Counterstain: DAPI. Staining: (A) DAPI. (B) Anti-EIF2A and DyLight™488. (C) merge of A+B. (D). Secondary only. Localization: cytosol, extracellular >> mitochondria. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          EIF2A Antibody in Immunocytochemistry (ICC/IF)
          EIF2A Antibody in Western Blot (WB)
          EIF2A Antibody in Western Blot (WB)
          EIF2A Antibody in ELISA (ELISA)
          EIF2A Polyclonal Antibody

          Product Details

          600-101-ML7

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          -

          Immunocytochemistry (ICC/IF)

          5 µg/mL
          -

          ELISA (ELISA)

          1:10,000-1:50,000
          -
          Product Specifications

          Species Reactivity

          Human, Mouse

          Host/Isotype

          Goat / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Anti-EIF2A antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to a near N-terminal portion of human EIF2A conjugated to Keyhole Limpet Hemocyanin (KLH).
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1.08 mg/mL

          Amount

          100 µg

          Purification

          Affinity chromatography

          Storage buffer

          0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

          Contains

          0.01% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          Product Specific Information

          Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

          This affinity purified antibody is directed against human EIF2A. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 86.7% homology with the immunizing sequence to mouse.

          Target Information

          EIF2A is a 65-kD protein that catalyzes the formation of puromycin-sensitive 80S preinitiation complexes.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: 65 kDa eukaryotic translation initiation factor 2A; eIF-2A; eIF2A; Eukaryotic translation initiation factor 2A; eukaryotic translation initiation factor 2A, 65kDa; unnamed protein product

          View more View less

          Gene Aliases: CDA02; D030048D22; D3Ertd194e; EIF-2A; EIF2A; MST089; MSTP004; MSTP089

          View more View less

          UniProt ID: (Human) Q9BY44, (Mouse) Q8BJW6

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          Entrez Gene ID: (Human) 83939, (Mouse) 229317

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          Function(s)
          tRNA binding mRNA binding translation initiation factor activity protein binding ribosome binding cadherin binding translation factor translation initiation factor translational protein
          Process(es)
          translation translational initiation regulation of translation positive regulation of signal transduction SREBP signaling pathway ribosome assembly response to amino acid starvation protein phosphorylation
          It has to be done as per old AB suggested Products section.

          Disclaimer

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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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