Invitrogen

EIF3J Polyclonal Antibody

View all (11) eIF3j antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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EIF3J Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of EIF3J in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and EIF3J polyclonal antibody (Product # PA5-103835) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).

EIF3J Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded EIF3J in human prostate tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with EIF3J polyclonal antibody (Product # PA5-103835) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

EIF3J Antibody in Western Blot (WB) — Western blot analysis of EIF3J in A2780 cell lysate (left lane: treated with blocking peptide). Samples were incubated with EIF3J polyclonal antibody (Product # PA5-103835).

Product Details

PA5-103835

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:3,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:200	-
Immunocytochemistry (ICC/IF)	1:100-1:500	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthesized peptide derived from human EIF3J(Accession O75822), corresponding to amino acid residues Q87-V107. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.4, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C
RRID	AB_2853168

Target Information

Eukaryotic initiation factor-3 (EIF3) has a molecular mass of about 600 kD and contains 13 nonidentical protein subunits, including EIF3J. EIF3 plays a central role in binding of initiator methionyl-tRNA and mRNA to the 40S ribosomal subunit to form the 40S initiation complex (Fraser et al., 2004; Fraser et al., 2007).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: eIF-3-alpha; eIF-3-alpha {ECO:0000255|HAMAP-Rule:MF_03009}; eIF-3-alpha-A; eIF-3-alpha-B; eIF3 p35; eIF3 p35 {ECO:0000255|HAMAP-Rule:MF_03009}; EIF3J; eIF3j {ECO:0000255|HAMAP-Rule:MF_03009}; eIF3j-A; eIF3j-B; Eukaryotic translation initiation factor 3 subunit 1; eukaryotic translation initiation factor 3 subunit 1 {ECO:0000255|HAMAP-Rule:MF_03009}; Eukaryotic translation initiation factor 3 subunit 1-A; Eukaryotic translation initiation factor 3 subunit 1-B; Eukaryotic translation initiation factor 3 subunit J; eukaryotic translation initiation factor 3 subunit J {ECO:0000255|HAMAP-Rule:MF_03009}; Eukaryotic translation initiation factor 3 subunit J-A; Eukaryotic translation initiation factor 3 subunit J-B; eukaryotic translation initiation factor 3, subunit 1 (alpha, 35kD); eukaryotic translation initiation factor 3, subunit 1 alpha; eukaryotic translation initiation factor 3, subunit J

Gene Aliases: 1810016I04Rik; 2700079K05Rik; AA409117; AA409446; C78575; eIF-3-alpha-A; eIF-3-alpha-B; eIF3 p35; eIF3-alpha; eIF3-p35; EIF3J; Eif3j-1; Eif3j-2; eIF3j-A; eIF3j-B; Eif3j1; Eif3j2; EIF3S1; Eif3s1-1; Eif3s1-2; ENSMUSG00000043424; Gm9781; PRO0391

UniProt ID: (Human) Q9H8Q2, (Rat) A0JPM9, (Mouse) Q3UGC7, (Mouse) Q66JS6

Entrez Gene ID: (Human) 8669, (Rat) 691947, (Mouse) 78655, (Mouse) 100042807

Molecular Function: translation initiation factor