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EIF4A2 Polyclonal Antibody

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Antibody Testing Data (9)

EIF4A2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 9

EIF4A2 Antibody (11280-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using (Product # 11280-1-AP) (EIF4A2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

EIF4A2 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

11280-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Pig

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

EIF4A2 Fusion Protein Ag1810 (108-407 aa encoded by BC015842)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: APTRELAQQ IQKVILALGD YMGATCHACI GGTNVRNEMQ KLQAEAPHIV VGTPGRVFDM LNRRYLSPKW IKMFVLDEAD EMLSRGFKDQ IYEIFQKLNT SIQVVLLSAT MPTDVLEVTK KFMRDPIRIL VKKEELTLEG IKQFYINVER EEWKLDTLCD LYETLTITQA VIFLNTRRKV DWLTEKMHAR DFTVSALHGD MDQKERDVIM REFRSGSSRV LITTDLLARG IDVQQVSLVI NYDLPTNREN YIHRIGRGGR FGRKGVAINF VTEEDKRILR DIETFYNTTV EEMPMNVADL I (108-407 aa encoded by BC015842)

Target Information

eIF4A2 is a 407 amino acid ATP-dependent RNA helicase belonging to the DEAD box helicase family. It contains one helicase ATP-binding domain and a helicase C-terminal domain. eIF4A2, a subunit of the eIF4F complex, acts as a protein-synthesis initiation factor and thus plays an important role in the binding of mRNA to the 43S pre-initiation complex of ribosomes. Human eIF4A2 interacts with the NS5B protein of Hepatitis C virus (HCV) and facilitates the genomic RNA synthesis of NS5B protein by unwinding the secondary structure of the HCV genome. It is ubiquitously expressed in most of normal tissues, but in variable amounts.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Local translation in nuclear condensate amyloid bodies.

Proceedings of the National Academy of Sciences of the United States of America

Theodoridis PR,Bokros M,Marijan D,Balukoff NC,Wang D,Kirk CC,Budine TD,Goldsmith HD,Wang M,Audas TE,Lee S

Tue Feb 16 00:00:00 EST 2021

Local translation in nuclear condensate amyloid bodies.

Proceedings of the National Academy of Sciences of the United States of America

Theodoridis PR,Bokros M,Marijan D,Balukoff NC,Wang D,Kirk CC,Budine TD,Goldsmith HD,Wang M,Audas TE,Lee S

Tue Feb 16 00:00:00 EST 2021

Identification of dynamic RNA-binding proteins uncovers a Cpeb4-controlled regulatory cascade during pathological cell growth of cardiomyocytes.

Cell reports

Riechert E,Kmietczyk V,Stein F,Schwarzl T,Sekaran T,Jürgensen L,Kamuf-Schenk V,Varma E,Hofmann C,Rettel M,Gür K,Ölschläger J,Kühl F,Martin J,Ramirez-Pedraza M,Fernandez M,Doroudgar S,Méndez R,Katus HA,Hentze MW,Völkers M

Tue May 11 00:00:00 EDT 2021

Translatome and Transcriptome Profiling of Hypoxic-Induced Rat Cardiomyocytes.

Molecular therapy. Nucleic acids

Shen Z,Zeng L,Zhang Z

Fri Dec 04 00:00:00 EST 2020

EIF4A2 interacts with the membrane protein of transmissible gastroenteritis coronavirus and plays a role in virus replication.

Research in veterinary science

Song Z,Yang Y,Wang L,Wang K,Ran L,Xie Y,Huang L,Yang Z,Yuan P,Yu Q

Mon Apr 01 00:00:00 EDT 2019

EIF4A2 interacts with the membrane protein of transmissible gastroenteritis coronavirus and plays a role in virus replication.

Research in veterinary science

Song Z,Yang Y,Wang L,Wang K,Ran L,Xie Y,Huang L,Yang Z,Yuan P,Yu Q

Mon Apr 01 00:00:00 EDT 2019

eIF4A2 is a host factor required for efficient HIV-1 replication.

Microbes and infection

Ndzinu JK,Takeuchi H,Saito H,Yoshida T,Yamaoka S

Fri May 03 00:00:00 EDT 2019

eIF4A2 is a host factor required for efficient HIV-1 replication.

Microbes and infection

Ndzinu JK,Takeuchi H,Saito H,Yoshida T,Yamaoka S

Fri May 03 00:00:00 EDT 2019

MiR-181a/b induce the growth, invasion, and metastasis of neuroblastoma cells through targeting ABI1.

Molecular carcinogenesis

Liu X,Peng H,Liao W,Luo A,Cai M,He J,Zhang X,Luo Z,Jiang H,Xu L

Sat Sep 01 00:00:00 EDT 2018

MiR-181a/b induce the growth, invasion, and metastasis of neuroblastoma cells through targeting ABI1.

Molecular carcinogenesis

Liu X,Peng H,Liao W,Luo A,Cai M,He J,Zhang X,Luo Z,Jiang H,Xu L

Sat Sep 01 00:00:00 EDT 2018

Nuclear localization of EIF4G3 suggests a role for the XY body in translational regulation during spermatogenesis in mice.

Biology of reproduction

Hu J,Sun F,Handel MA

Mon Jan 01 00:00:00 EST 2018

Nuclear localization of EIF4G3 suggests a role for the XY body in translational regulation during spermatogenesis in mice.

Biology of reproduction

Hu J,Sun F,Handel MA

Mon Jan 01 00:00:00 EST 2018

Nuclear localization of EIF4G3 suggests a role for the XY body in translational regulation during spermatogenesis in mice.

Biology of reproduction

Hu J,Sun F,Handel MA

Mon Jan 01 00:00:00 EST 2018

Nuclear localization of EIF4G3 suggests a role for the XY body in translational regulation during spermatogenesis in mice.

Biology of reproduction

Hu J,Sun F,Handel MA

Mon Jan 01 00:00:00 EST 2018

Bioinformatics

Protein Aliases: ATP dependent RNA helicase eIF4A 2; ATP-dependent RNA helicase eIF4A-2; eIF-4A-II; eIF4A-II; Eukaryotic initiation factor 4A-II; eukaryotic translation initiation factor 4A isoform 2; eukaryotic translation initiation factor 4A, isoform 2; N-terminally processed; protein synthesis initiation factor 4AII

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Gene Aliases: 4833432N07Rik; BM-010; DDX2B; eIF-4A-II; Eif4; EIF4A; eIF4A-II; EIF4A2; EIF4F

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UniProt ID: (Human) Q14240, (Mouse) P10630, (Rat) Q5RKI1

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Entrez Gene ID: (Human) 1974, (Mouse) 13682, (Rat) 303831

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Function(s)

translation initiation factor activity ATP-dependent RNA helicase activity helicase activity protein binding ATP binding ATPase activity poly(A) RNA binding nucleotide binding nucleic acid binding RNA binding hydrolase activity

Process(es)

nuclear-transcribed mRNA poly(A) tail shortening translational initiation regulation of translational initiation RNA secondary structure unwinding viral process negative regulation of RNA-directed RNA polymerase activity translation regulation of gene expression

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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EIF4A2 Polyclonal Antibody

EIF4A2 Antibody (11280-1-AP) in IHC (P)

Product Details

11280-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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