Rockland

EIF4E Polyclonal Antibody

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Datasheet

Protocols

Questions & Answers

Tech Support

Product Details

200-901-MK9

Applications	Tested Dilution	Publications
Western Blot (WB)	5 µg/mL	-
Immunohistochemistry (IHC)	1:1,000	-
ELISA (ELISA)	Assay-dependent	-

Product Specifications
Species Reactivity	Human, Mouse
Host/Isotype	Chicken / IgY
Class	Polyclonal
Type	Antibody
Immunogen	Anti-EIF4E antibody was prepared from eggs of chickens laid after repeated immunizations with a synthetic peptide corresponding to a near C-Terminal portion of human EIF4E conjugated to Keyhole Limpet Hemocyanin (KLH). View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1.05 mg/mL
Purification	selective precipitation
Storage buffer	0.002M sodium phosphate, pH 7, with 0.15M NaCl
Contains	0.01% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This affinity purified antibody is directed against human EIF4E. This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above.A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to bovine and 93% homology to rat, mouse, and rabbit.

Target Information

eIF4E is involved in several cellular processes including enhanced translational efficiency, splicing, mRNA stability, and RNA nuclear export. All eukaryotic cellular mRNAs are blocked at their 5-prime ends with the 7-methylguanosine cap structure, m7GpppX (where X is any nucleotide). EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A, that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: eIF-4E; eIF-4F 25 kDa subunit; Eukaryotic translation initiation factor 4E; eukaryotic translation initiation factor 4E-like 1; MGC111573; mRNA cap-binding protein; OTTHUMP00000219703; OTTHUMP00000219704; OTTHUMP00000219705

Gene Aliases: AUTS19; CBP; EG668879; eIF-4E; EIF4E; Eif4e-ps; EIF4E1; EIF4EL1; EIF4F; If4e

UniProt ID: (Human) P06730, (Mouse) P63073

Entrez Gene ID: (Human) 1977, (Mouse) 13684

Molecular Function: translation factor translation initiation factor translational protein