Proteintech

EMG1 Polyclonal Antibody, CoraLite® Plus 488

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Protocols
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Datasheet
Protocols
Questions & Answers
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Product Details

CL488-11965

Applications
Tested Dilution
Publications

Immunocytochemistry (ICC/IF)

1:50-1:500
-

Flow Cytometry (Flow)

0.80 µg/10^6 cells in a 100 µL suspension
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

EMG1 fusion protein Ag2575

Conjugate

CoraLite® Plus 488 CoraLite® Plus 488 CoraLite® Plus 488

Excitation/Emission Max

493/522 nm

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 0.5% BSA, 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20°C, store in dark

Shipping conditions

Wet ice

Product Specific Information

Immunogen sequence: MAAPSDGFK PRERSGGEQA QDWDALPPKR PRLGAGNKIG GRRLIVVLEG ASLETVKVGK TYELLNCDKH KSILLKNGRD PGEARPDITH QSLLMLMDSP LNRAGLLQVY IHTQKNVLIE VNPQTRIPRT FDRFCGLMVQ LLHKLSVRAA DGPQKLLKVI KNPVSDHFPV GCMKVGTSFS IPVVSDVREL VPSSDPIVFV VGAFAHGKVS VEYTEKMVSI SNYPLSAALT CAKLTTAFEE VWGVI

Target Information

EMG1 involved in 40S ribosomal subunit biogenesis. It seems to play a role a methylation reaction in pre-rRNA processing.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 18S rRNA (pseudouridine(1248)-N1)-methyltransferase; 18S rRNA (pseudouridine-N1-)-methyltransferase NEP1; 18S rRNA Psi1248 methyltransferase; EMG1 N1-specific pseudouridine methyltransferase; EMG1 nucleolar protein homolog; essential for mitotic growth 1; gene rich cluster, C2f; nucleolar essential protein 1; Nucleolar protein EMG1 homolog; probable ribosome biogenesis protein NEP1; Protein C2f; Ribosomal RNA small subunit methyltransferase NEP1; Ribosome biogenesis protein NEP1

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Gene Aliases: C2F; EMG1; Grcc2f; NEP1

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UniProt ID: (Human) Q92979, (Mouse) O35130

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Entrez Gene ID: (Human) 10436, (Mouse) 14791, (Rat) 312706

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Function(s)
RNA binding protein binding rRNA binding poly(A) RNA binding rRNA (pseudouridine) methyltransferase activity rRNA (adenine-N6,N6-)-dimethyltransferase activity protein C-terminal carboxyl O-methyltransferase activity tRNA (guanine-N2-)-methyltransferase activity methyltransferase activity C-methyltransferase activity N-methyltransferase activity O-methyltransferase activity S-methyltransferase activity RNA methyltransferase activity mRNA methyltransferase activity tRNA methyltransferase activity protein methyltransferase activity 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity rRNA methyltransferase activity rRNA (uridine-2'-O-)-methyltransferase activity rRNA (adenine-N6-)-methyltransferase activity DNA-methyltransferase activity tRNA (guanine-N1-)-methyltransferase activity rRNA (cytosine-C5-)-methyltransferase activity selenocysteine methyltransferase activity arginine N-methyltransferase activity protein-arginine N-methyltransferase activity lysine N-methyltransferase activity protein-lysine N-methyltransferase activity tRNA (uracil) methyltransferase activity tRNA (guanine) methyltransferase activity tRNA (adenine) methyltransferase activity tRNA (cytosine) methyltransferase activity tRNA (cytosine-5-)-methyltransferase activity tRNA (adenine-N1-)-methyltransferase activity rRNA (adenine) methyltransferase activity rRNA (cytosine) methyltransferase activity rRNA (guanine) methyltransferase activity rRNA (uridine) methyltransferase activity transferase activity protein C-terminal leucine carboxyl O-methyltransferase activity 1-phenanthrol methyltransferase activity protein-arginine N5-methyltransferase activity methylarsonite methyltransferase activity dimethylarsinite methyltransferase activity 4,5-dihydroxybenzo(a)pyrene methyltransferase activity 1-hydroxypyrene methyltransferase activity 1-hydroxy-6-methoxypyrene methyltransferase activity demethylmenaquinone methyltransferase activity cobalt-precorrin-6B C5-methyltransferase activity cobalt-precorrin-7 C15-methyltransferase activity cobalt-precorrin-5B C1-methyltransferase activity cobalt-precorrin-3 C17-methyltransferase activity dimethylamine methyltransferase activity hydroxyneurosporene-O-methyltransferase activity tRNA (adenine-57, 58-N(1)-) methyltransferase activity methylamine-specific methylcobalamin:coenzyme M methyltransferase activity trimethylamine methyltransferase activity methanol-specific methylcobalamin:coenzyme M methyltransferase activity monomethylamine methyltransferase activity P-methyltransferase activity Se-methyltransferase activity 2-phytyl-1,4-naphthoquinone methyltransferase activity tRNA (uracil-2'-O-)-methyltransferase activity tRNA (cytosine-2'-O-)-methyltransferase activity phosphomethylethanolamine N-methyltransferase activity tRNA (cytosine-3-)-methyltransferase activity rRNA (cytosine-2'-O-)-methyltransferase activity rRNA (cytosine-N4-)-methyltransferase activity trihydroxyferuloyl spermidine O-methyltransferase activity methyltransferase
Process(es)
maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) rRNA processing ribosomal small subunit biogenesis rRNA base methylation blastocyst development nucleologenesis rRNA methylation methylation ribosome biogenesis
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