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EN1/Engrailed 1 Polyclonal Antibody
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FIGURE: 1 / 1
EN1/Engrailed 1 Antibody (BS-11744R) in Flow
Blank control (blue): Raji Cells (fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-Engrailed 1 AF647 Conjugated antibody (bs-11744R-A647), Dilution: 0.2 µg in 100 µL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.
Product Details
BS-11744R
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide derived from human EN1/Engrailed 1, amino acids 351-392.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol
Contains
0.02% ProClin 300
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
EN1 (Engrailed homolog 1) is a transcription factor expressed in embryonic ventral limb ectoderm and is essential for ventral limb patterning. En1 expression in the developing perichordal tube suggests that it may also be involved in vertebrae assembly. Loss of EN1 function in mice results in dorsal transformations of ventral paw structures and in subtle alterations along the proximal/distal limb axis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: engrailed 1; engrailed homeobox 1; engrailed homolog 1; engrailed-1; HME1; Homeobox protein en-1; Homeobox protein engrailed-1; hu-En-1; mo-En-1
Gene Aliases: En-1; EN1; ENDOVESLB; Mo-en.1
UniProt ID: (Human) Q05925, (Mouse) P09065
Entrez Gene ID: (Human) 2019, (Mouse) 13798, (Rat) 685360
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
sequence-specific double-stranded DNA binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
protein binding
sequence-specific DNA binding
molecular_function
homeodomain transcription factor
negative regulation of transcription from RNA polymerase II promoter
skeletal system development
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
adult locomotory behavior
anatomical structure morphogenesis
dorsal/ventral pattern formation
proximal/distal pattern formation
regulation of gene expression
cerebellum development
central nervous system neuron differentiation
neuron differentiation
embryonic limb morphogenesis
midbrain development
hindbrain development
midbrain-hindbrain boundary development
embryonic forelimb morphogenesis
social behavior
multicellular organism growth
response to cocaine
drinking behavior
pigmentation
negative regulation of neuron apoptotic process
positive regulation of transcription from RNA polymerase II promoter
neuron development
limb development
motor learning
dopaminergic neuron differentiation
embryonic brain development
transcription from RNA polymerase II promoter
multicellular organism development
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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