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          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • EN2 Antibodies

          Invitrogen

          EN2 Polyclonal Antibody

          View all (7) EN2 antibodies

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          Cite EN2 Polyclonal Antibody

          • Antibody Testing Data (3)
          EN2 Antibody in Immunocytochemistry (ICC/IF)
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          EN2 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          EN2 Antibody (PA5-97706) in ICC/IF

          Immunofluorescent analysis of EN2 in Hela cells using a EN2 polyclonal antibody (Product # PA5-97706) at a dilution of 1:100. Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L) secondary antibody was used. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          EN2 Antibody in Immunocytochemistry (ICC/IF)
          EN2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          EN2 Antibody in Western Blot (WB)
          EN2 Polyclonal Antibody

          Product Details

          PA5-97706

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:5,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:20-1:200
          -

          Immunocytochemistry (ICC/IF)

          1:50-1:200
          -

          ELISA (ELISA)

          Assay-Dependent
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant Human Homeobox protein engrailed-2 protein (1-251AA)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          3.5 mg/mL

          Purification

          Protein G

          Storage buffer

          PBS, pH 7.4, with 50% glycerol

          Contains

          0.03% ProClin 300

          Storage conditions

          -20°C or -80°C if preferred

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2812321

          Target Information

          Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Let us know so we can reference it here.
          Cite this product

          Bioinformatics

          Protein Aliases: AUTS1; engrailed homolog 2; engrailed-2; Homeobox protein en-2; Homeobox protein engrailed-2; hu-En-2

          View more View less

          Gene Aliases: EN2

          View more View less

          UniProt ID: (Human) P19622

          View more View less

          Entrez Gene ID: (Human) 2020

          View more View less

          Function(s)
          sequence-specific DNA binding homeodomain transcription factor
          Process(es)
          multicellular organism development midbrain development hindbrain development negative regulation of neuron apoptotic process positive regulation of transcription from RNA polymerase II promoter neuron development embryonic brain development
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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