Invitrogen

ENPP1 Polyclonal Antibody

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ENPP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of ENPP1 in paraffin-embedded Rat Intestine tissue. Samples were incubated with polyclonal antibody (Product # PA5-119090) at a dilution of 20 µg/mL, followed by HRP-Linked Caprine Anti-Rabbit IgG using a dilution of 2 µg/mL.

ENPP1 Antibody in Western Blot (WB) — Western blot analysis of ENPP1 in Rat Liver lysate. Samples were incubated with polyclonal antibody (Product # PA5-119090) at a dilution of 1 µg/mL, followed by HRP-Linked Caprine Anti-Rabbit IgG using a dilution of 0.2 µg/mL.

Product Details

PA5-119090

Applications	Tested Dilution	Publications
Western Blot (WB)	0.2-2 µg/mL	-
Immunohistochemistry (Paraffin) (IHC (P))	5-20 µg/mL	-
Immunocytochemistry (ICC/IF)	5-20 µg/mL	-

Product Specifications
Species Reactivity	Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein Ectonucleotide Pyrophosphatase/Phosphodiesterase 1
Conjugate	Unconjugated
Form	Liquid
Concentration	500 µg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS with 50% glycerol
Contains	0.05% Proclin 300
Storage conditions	-20°C

Target Information

This gene is a member of the ecto-nucleotide pyrophosphatase/phosphodiesterase (ENPP) family. The encoded protein is a type II transmembrane glycoprotein comprising two identical disulfide-bonded subunits. This protein has broad specificity and cleaves a variety of substrates, including phosphodiester bonds of nucleotides and nucleotide sugars and pyrophosphate bonds of nucleotides and nucleotide sugars. This protein may function to hydrolyze nucleoside 5' triphosphates to their corresponding monophosphates and may also hydrolyze diadenosine polyphosphates. Mutations in this gene have been associated with 'idiopathic' infantile arterial calcification, ossification of the posterior longitudinal ligament of the spine (OPLL), and insulin resistance.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Alkaline phosphodiesterase I; E-NPP 1; Ectonucleotide pyrophosphatase/phosphodiesterase family member 1; Ectonucleotide pyrophosphatase/phosphodiesterase family member 1, secreted form; HGNC:3356; NPPase; Nucleotide diphosphatase; Nucleotide pyrophosphatase; OTTHUMP00000043194; Phosphodiesterase I/nucleotide pyrophosphatase 1; Plasma-cell membrane glycoprotein PC-1

Gene Aliases: Enpp1; Npps; Pc1; Pdnp1

UniProt ID: (Rat) Q924C3

Entrez Gene ID: (Rat) 85496

Function(s)