Proteintech

ERI1 Polyclonal Antibody

View all (10) ERI1 antibodies

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ERI1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells using 14592-1-AP (ERI1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

ERI1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver cancer using 14592-1-AP (ERI1 antibody) at dilution of 1:100 (under 10x lens).

ERI1 Antibody in Western Blot (WB) — HepG2 cells were subjected to SDS PAGE followed by western blot with 14592-1-AP (ERI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Product Details

14592-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:10-1:100	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ERI1 Fusion Protein Ag6125 (1-349 aa encoded by BC035279) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MEDPQSKEP AGEAVALALL ESPRPEGGEE PPRPSPEETQ QCKFDGQETK GSKFITSSAS DFSDPVYKEI AITNGCINRM SKEELRAKLS EFKLETRGVK DVLKKRLKNY YKKQKLMLKE SNFADSYYDY ICIIDFEATC EEGNPPEFVH EIIEFPVVLL NTHTLEIEDT FQQYVRPEIN TQLSDFCISL TGITQDQVDR ADTFPQVLKK VIDWMKLKEL GTKYKYSLLT DGSWDMSKFL NIQCQLSRLK YPPFAKKWIN IRKSYGNFYK VPRSQTKLTI MLEKLGMDYD GRPHCGLDDS KNIARIAVRM LQDGCELRIN EKMHAGQLMS VSSSLPIEGT PPPQMPHFRK (1-349 aa encoded by BC035279)

Target Information

RNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. A 2' and 3'-hydroxyl groups at the last nucleotide of the histone 3'-end is required for efficient degradation of RNA substrates. Also able to degrade the 3'-overhangs of short interfering RNAs (siRNAs) in vitro, suggesting a possible role as regulator of RNA interference (RNAi). Requires for binding the 5'-ACCCA-3' sequence present in stem-loop structure. Able to bind other mRNAs. Required for 5.8S rRNA 3'-end processing. Also binds to 5.8s ribosomal RNA. Binds with high affinity to the stem-loop structure of replication-dependent histone pre-mRNAs.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 3 ' exoribonuclease; 3' exoribonuclease; 3'-5' exonuclease ERI1; 3'-5' exoribonuclease 1; enhanced RNAi protein 1; enhanced RNAi three prime mRNA exonuclease homolog 1; Eri-1 homolog; HEXO; histone mRNA 3' end-specific exonuclease; Histone mRNA 3'-end-specific exoribonuclease; Histone mRNA 3'-exonuclease 1; protei; Protein 3'hExo; three prime histone mRNA exonuclease 1; three prime mRNA exonuclease 1

Gene Aliases: 3'EXO; 3'HEXO; 3110010F15Rik; eri-1; ERI1; HEXO; RGD1308378; THEX1

UniProt ID: (Human) Q8IV48, (Rat) Q5FVR4, (Mouse) Q9CQ63

Entrez Gene ID: (Human) 90459, (Rat) 361159, (Mouse) 67276

Molecular Function: exoribonuclease