|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues I(490) Q E E K P K K K K K A Q E D L(505) of human ERp57.|
|Storage buffer||whole serum|
|Contains||0.01% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay dependent|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunoprecipitation (IP)||See 1 publications below|
PA3-009 detects ERp57 protein in human samples.
PA3-009 has successfully been used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects a 57 kDa protein representing ERp57 in rat liver homogenate.
The PA3-009 immunogen is a synthetic peptide corresponding to residues I(490) Q E E K P K K K K K A Q E D L(505) of human ERp57. This peptide (Cat. # PEP-225) is available for use in neutralization and control experiments.
ERp57, also known as GRP58, was cloned by three independent labs, all of which reported that the gene encodes a 505-amino acid protein that shares significant homology with the molecular chaperone protein disulfide isomerase (PDI). The ERp57 sequence was also shown to include a putative nuclear localization motif and an endoplasmic reticulum (ER) retention motif. Studies have shown that ERp57 functions together with calnexin and calreticulin as a molecular chaperone of glycoprotein biosynthesis within the ER.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Tumor necrosis factor-¿ treatment of HepG2 cells mobilizes a cytoplasmic pool of ERp57/1,25D¿-MARRS to the nucleus.
PA3-009 was used in immunoprecipitation to study the nuclear translocation of cytoplasmic ERp57
|Grindel BJ,Rohe B,Safford SE,Bennett JJ,Farach-Carson MC||Journal of cellular biochemistry (112:2606)||2011|