Invitrogen

ETHE1 Polyclonal Antibody

ETHE1 Antibody in Immunocytochemistry (ICC/IF)

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ETHE1 Antibody (PA5-56040) in ICC/IF

Immunofluorecent analysis of ETHE1 in human cell line U-2 OS using ETHE1 Polyclonal Antibody (Product # PA5-56040). Staining shows localization to nucleoplasm and mitochondria.

ETHE1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ETHE1 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

ETHE1 Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of ETHE1 demonstrates antibody specificity. Immunohistochemical analysis of ETHE1 using anti-ETHE1 Polyclonal Antibody (Product # PA5-56040), shows significant staining of ETHE1 in human colon and shows minimal or weak staining in human testis tissues. The relative expression levels of ETHE1 within each tissue is shown using RNA-Seq. {RE}

Product Details

PA5-56040

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000-1:2,500

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human ETHE1. Recombinant protein control fragment (Product #RP-95106).

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.30 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2641135

Product Specific Information

Immunogen sequence: RTDFQQGCAK TLYHSVHEKI FTLPGDCLIY PAHDYHGFTV STVEEERTLN PRLTLSCEEF VKIMGNLNLP KPQQIDFAVP ANMRCG

Highest antigen sequence identity to the following orthologs: Mouse - 93%, Rat - 92%.

Target Information

ETHE1 is a sulfur dioxygenase that localizes within the mitochondrial matrix. The enzyme functions in sulfide catabolism. Mutations in its gene result in ethylmalonic encephalopathy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CTH/MPST double ablation results in enhanced vasorelaxation and reduced blood pressure via upregulation of the eNOS/sGC pathway.

Frontiers in pharmacology

Katsouda A,Markou M,Zampas P,Varela A,Davos CH,Vellecco V,Cirino G,Bucci M,Papapetropoulos A

Published figure using ETHE1 polyclonal antibody (Product # PA5-56040) in Western Blot

Fri Mar 03 00:00:00 UTC 2023

Hydrogen sulfide as an anti-calcification stratagem in human aortic valve: Altered biogenesis and mitochondrial metabolism of H₂S lead to H₂S deficiency in calcific aortic valve disease.

Redox biology

Combi Z,Potor L,Nagy P,Sikura KÉ,Ditrói T,Jurányi EP,Galambos K,Szerafin T,Gergely P,Whiteman M,Torregrossa R,Ding Y,Beke L,Hendrik Z,Méhes G,Balla G,Balla J

Published figure using ETHE1 polyclonal antibody (Product # PA5-56040) in Western Blot

Sat Apr 01 00:00:00 UTC 2023

Hydrogen sulfide, oxygen, and calcium regulation in developing human airway smooth muscle.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Bartman CM,Schiliro M,Helan M,Prakash YS,Linden D,Pabelick C

PA5-56040 was used in Western Blotting to provide novel information regarding the potential for H2 S to act as a bronchodilator in developing airways in the context of oxygen exposure.

Tue Sep 01 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: ethylmalonic encephalopathy 1; Ethylmalonic encephalopathy protein 1; Ethylmalonic encephalopathy protein 1 homolog; Hepatoma subtracted clone one protein; Persulfide dioxygenase ETHE1, mitochondrial; protein ETHE1, mitochondrial; Sulfur dioxygenase ETHE1

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Gene Aliases: 0610025L15Rik; ETHE1; HSCO; YF13H12

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UniProt ID: (Human) O95571, (Mouse) Q9DCM0

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Entrez Gene ID: (Human) 23474, (Mouse) 66071, (Rat) 292710

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Function(s)

iron ion binding sulfur dioxygenase activity oxidoreductase activity metal ion binding dioxygenase activity oxygenase

Process(es)

glutathione metabolic process sulfide oxidation, using sulfide:quinone oxidoreductase hydrogen sulfide metabolic process oxidation-reduction process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ETHE1 Polyclonal Antibody

ETHE1 Antibody (PA5-56040) in ICC/IF

Product Details

PA5-56040

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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