Invitrogen

ETV1 Polyclonal Antibody

Product Image Gallery

ETV1 Antibody — Figure 4 miR-34b target genes and luciferase reporter assay A. qPCR analysis of AR, BCL2, ETV1, PDPK1 and IGF1 from MDA-PCa-2b or DU-145 cells transfected with miR-34b-3p mimic. Asterisks indicate a p-value <0.05, calculated by two-tailed t-test. B. ETV1, IGF1 and AR levels were assessed by Western blot analysis. C. miR-34b binding sites in AR and ETV1 3 and apos;-UTR mediate the down-regulation of AR and ETV1 protein expression by miR-34b. p-value was calculated by two-tailed t-test.

ETV1 Antibody in Immunohistochemistry (IHC) — Immunohistochemistry analysis of brain, cortex tissue using an anti-ETV1 polyclonal antibody (Product # PA5-41484).

ETV1 Antibody in Western Blot (WB) — Western blot analysis of HEK293 cell lysate using an anti-ETV1 polyclonal antibody (Product # PA5-41484). Primary Antibody Dilution: 1:1000; Secondary Antibody: Anti-rabbit-HRP. LANE 1) 100µg untransfected HEK293 lysate; LANE 2) 33µg ER81 transfected HEK293 lysate.

Product Details

Tested Applications

Dilution

Immunohistochemistry (IHC)

5 µg/mL

Western Blot (WB)

1:1000

Published Applications

Western Blot (WB)

See 2 publications below

Product Specifications

Species Reactivity

Bovine, Dog, Horse, Guinea pig, Human, Mouse, Rabbit, Rat, Zebrafish

Published species

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide directed towards the middle region of human ETV1

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS with 2% sucrose

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2608631

Product Specific Information

Peptide sequence: PDNQRPLLKT DMERHINEED TVPLSHFDES MAYMPEGGCC NPHPYNEGYV

Sequence homology: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 86%; Rat: 100%; Zebrafish: 86%

Target Information

ETV1: ets variant gene 1. Several members of the Ets gene family encode sequence-specific DNA binding proteins that recognize DNA sequences with a centrally located 5'-GGAA-3' element. All of the Ets proteins recognize the same central core sequence but each protein interacts with unique sequences that flank this core. ER81 (also designated ETV1) binds the motif 5'-CGGAA/T-3'. ER81 is highly expressed in brain, testis, lung and heart. ER81 is also moderately expressed in spleen, pancreas, colon and small intestine. During development, ER81 displays a unique expression patterns which suggests that this transcriptional factor might play an important role in organogenesis. ERK-1 activates ER81 transcriptional activity while MAPKAP kinase 2 inhibits ER81.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: DKFZp781L0674; ER81; ets related protein 81; ETS transcription factor Er81; ETS translocation variant 1; Ets variant 1; ets variant gene 1; ets variant gene 1-like; Ets-related protein 81; MGC104699; MGC120533; MGC120534

Gene Aliases: BOS_4062; ER81; Etsrp81; ETV1

UniProt ID: (Bovine) Q2KIC2, (Human) P50549, (Mouse) P41164

Entrez Gene ID: (Dog) 475246, (Bovine) 540846, (Human) 2115, (Rat) 362733, (Guinea pig) 100721423, (Mouse) 14009, (Rabbit) 100355978, (Zebrafish) 563876

Molecular Function: nucleic acid binding signaling molecule transcription factor