Proteintech

EXOSC10 Polyclonal Antibody

Product Image Gallery

EXOSC10 Antibody in Western Blot (WB) — WB result of EXOSC10 antibody (11178-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EXOSC10 transfected HeLa cells.

EXOSC10 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer using 11178-1-AP (EXOSC10 antibody) at dilution of 1:50 (under 40x lens).

EXOSC10 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of NIH/3T3 cells using 11178-1-AP (EXOSC10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

EXOSC10 Antibody in Immunoprecipitation (IP) — IP result of anti-EXOSC10 (IP:11178-1-AP, 4ug; Detection:11178-1-AP 1:300) with MCF-7 cells lysate 3200 ug.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC)

1:20-1:200

Immunofluorescence (IF)

1:20-1:200

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

EXOSC10 Fusion Protein Ag1666

Conjugate

Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.1% sodium azide

Storage conditions

-20°C

Target Information

About 50% of patients with polymyositis/scleroderma (PM-Scl) overlap syndrome are reported to have autoantibodies to a nuclear/nucleolar particle termed PM-Scl. Exosome component 10 (EXOSC10), also named autoantigen PM/Scl-2, is the 100 kDa antigen component of PM-Scl and is recognized by most sera of PM-Scl patients. EXOSC10 is strongly enriched in the nucleolus and a small amount has been found in cytoplasm supporting the existence of a nucleolar RNA exosome complex form. As a putative catalytic component ofthe RNA exosome complex which has 3'->5' exoribonuclease activity, EXOSC10 participates in a multitude of cellular RNA processing and degradation events.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: autoantigen PM-SCL; Autoantigen PM/Scl 2; Autoantigen PM/Scl 2 homolog; Exosome component 10; P100 polymyositis-scleroderma overlap syndrome-associated autoantigen; PM/Scl-100; Polymyositis/scleroderma autoantigen 100 kDa; Polymyositis/scleroderma autoantigen 2; Polymyositis/scleroderma autoantigen 2 homolog

Gene Aliases: EXOSC10; p2; p3; p4; PM-Scl; PM/Scl-100; PMSCL; PMSCL2; RRP6; Rrp6p

UniProt ID: (Human) Q01780, (Mouse) P56960

Entrez Gene ID: (Human) 5394, (Mouse) 50912

Molecular Function: RNA binding protein exoribonuclease hydrolase nuclease nucleic acid binding