Invitrogen

EpoR Polyclonal Antibody

View all (25) EpoR antibodies

Create Citation

Questions & Answers

Create Citation

Questions & Answers

Product Image Gallery

EpoR Antibody in Western Blot (WB) — Western blot analysis of EpoR in Lane 1: rat lung tissue lysates. Electrophoresis was performed with 5-20% SDS-PAGE gel (70V, Stacking gel; 90V Resolving gel, Time: 2-3 hours), transferred to a nitrocellulose membrane and blocked using 5% Non-fat Milk/TBS (1.5 hrs at room temperature). Samples were incubated with EpoR polyclonal antibody (Product # PA5-95480) using a 0.5 µg/mL dilution, followed by a goat anti-rabbit IgG-HRP at a dilution of 1:10,000, and developed with enhanced chemiluminescence (ECL).

Product Details

Applications

Tested Dilution

Publications

ELISA (ELISA)

0.1-0.5 µg/mL

-

Western Blot (WB)

0.1-0.5 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived human EPO Receptor recombinant protein (Position: E48-E226).

Conjugate

Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Affinity chromatography

Storage buffer

0.2mg sodium phosphate with 4mg trehalose, 0.9mg NaCl

Contains

0.05mg sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2807282

Product Specific Information

Reconstitution information: Add 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Target Information

Recombinant Human Erythropoietin Receptor (EPO R) is a type I membrane-spanning protein, expressed in a mouse myeloma cell line, NSO. This product is produced from a cDNA sequence encoding the extracellular domain of the EPO receptor. The 225 amino acid residue mature recombinant soluble EPO receptor, generated after cleavage of a 25 amino acid residue signal peptide, has predicted molecular mass of approximately 25 kDa.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this antibody

Bioinformatics

Protein Aliases: EPO-R; EPOR; Erythropoietin receptor

Gene Aliases: EPO-R; EPOR

UniProt ID: (Human) P19235, (Mouse) P14753

Entrez Gene ID: (Human) 2057, (Mouse) 13857, (Rat) 24336

Molecular Function: cytokine receptor receptor type I cytokine receptor

signal transduction positive regulation of cytosolic calcium ion concentration brain development heart development positive regulation of cell proliferation positive regulation of neuron projection development erythropoietin-mediated signaling pathway negative regulation of neuron apoptotic process decidualization positive regulation of ERK1 and ERK2 cascade negative regulation of hydrogen peroxide-induced cell death heart morphogenesis embryo development viral process cardiac muscle tissue morphogenesis ventricular cardiac muscle tissue morphogenesis vasculogenesis involved in coronary vascular morphogenesis visceral serous pericardium development

Process(es)

erythropoietin receptor activity protein binding identical protein binding transmembrane signaling receptor activity cytokine receptor activity