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Invitrogen
ErbB3 Polyclonal Antibody
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FIGURE: 1 / 3
ErbB3 Antibody (PA1-9040) in ICC/IF
Immunocytochemistry analysis of ErbB3 using ErbB3 Polyclonal Antibody (Product # PA1-9040) in paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing plasma membrane/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Product Details
PA1-9040
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to the C terminus amino acids HSRLFPKANAQRT (aa 1330-1342).
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with bovine, canine, mouse, porcine and rat based on sequence homology.
This antibody is tested in Peptide ELISA: antibody detection limit dilution 32,000.
Target Information
ErbB3 (c-erbB-3/HER-3) is a member of the type I family of growth factor receptors and binds to ligands in the heregulin family. ErbB3 is over-expressed in a variety of tumors in the prostate, bladder, breast, stomach, pancreas, and colon. Heregulin and EGF stimulate tyrosine phosphorylation of c-erbB-3 to different extents. The ErbB3 gene is located on the long arm of human chromosome 12 (12q13) and is transcribed into a 6. 2kb mRNA which is translated into a 160/180 kDa glycoprotein. The ErbB3 gene encodes a transmembrane receptor tyrosine kinase due to alternative splicing and forms heterodimers with other EGF receptor family members that have kinase activity. Heterodimerization leads to the activation of pathways that lead to cell proliferation or differentiation. Alternate transcriptional splice variants encoding different isoforms of ErbB3 have been characterized. One ErbB3 isoform lacks the intermembrane region, is secreted outside the cell, and acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-errB-3; epidermal growth factor receptor precursor; HER3 protein precursor; human epidermal growth factor receptor 3; MDA BF 1; MGC88033; p180 ErbB3; p45 sErbB3; p85 sErbB3; Proto-oncogene-like protein c-ErbB-3; Receptor tyrosine-protein kinase erbB-3; Tyrosine kinase-type cell surface receptor HER3; unnamed protein product; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3
Gene Aliases: c-erbB-3; c-erbB3; ErbB-3; ERBB3; erbB3-S; FERLK; HER3; LCCS2; MDA-BF-1; p180-ErbB3; p45-sErbB3; p85-sErbB3; VSCN1
UniProt ID: (Human) P21860
Entrez Gene ID: (Human) 2065
nucleotide binding
protein kinase activity
protein tyrosine kinase activity
transmembrane signaling receptor activity
protein binding
ATP binding
kinase activity
transferase activity
growth factor binding
protein tyrosine kinase activator activity
ubiquitin protein ligase binding
signaling receptor activity
neuregulin receptor activity
neuregulin binding
identical protein binding
ErbB-3 class receptor binding
protein heterodimerization activity
endocardial cushion development
negative regulation of cell adhesion
signal transduction
transmembrane receptor protein tyrosine kinase signaling pathway
epidermal growth factor receptor signaling pathway
peripheral nervous system development
heart development
negative regulation of signal transduction
positive regulation of gene expression
Schwann cell differentiation
Schwann cell development
cranial nerve development
neuron differentiation
ERBB2-ERBB3 signaling pathway
wound healing
regulation of cell proliferation
myelination
negative regulation of apoptotic process
positive regulation of MAPK cascade
protein kinase B signaling
negative regulation of neuron apoptotic process
positive regulation of epithelial cell proliferation
negative regulation of secretion
neuron apoptotic process
positive regulation of protein kinase B signaling
positive regulation of cardiac muscle tissue development
positive regulation of calcineurin-NFAT signaling cascade
motor neuron apoptotic process
extrinsic apoptotic signaling pathway in absence of ligand
negative regulation of motor neuron apoptotic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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