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Flow cytometry analysis of FAK was done on A549 cells treated with EGF (200ng/mL, 10 minutes). Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with FAK Rabbit Polyclonal Antibody (AHO0502, red histogram) or with rabbit isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA. After incubation at room temperature for 2 hours, the cells were labeled with Alexa Fluor® 488 Goat Anti-Rabbit Secondary Antibody (A11008) at a dilution of 1:400 for 30 minutes at room temperature. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.
|Tested species reactivity||Mouse , Human , Rat|
|Published species reactivity||Mouse , Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein corresponding to amino acid residues 853-1052 from theC-terminal region of human FAK.|
|Storage buffer||PBS, pH 7.3, with 0.2% BSA|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/million cells|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 5 publications below|
Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. Tyrosine 397 is the autophosphorylation site of FAK, and involved in its initial activation. This phosphorylated site binds Src family SH2 domains and the p85 subunit of PI3-Kinase, and activates cell migration and invasion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
MicroRNA miR-125a-3p modulates molecular pathway of motility and migration in prostate cancer cells.
AHO0502 was used in western blot to study miR-125a-3p in prostate cancer cells
|Ninio-Many L,Grossman H,Levi M,Zilber S,Tsarfaty I,Shomron N,Tuvar A,Chuderland D,Stemmer SM,Ben-Aharon I,Shalgi R||Oncoscience (1:250)||2015|
microRNA-125a-3p reduces cell proliferation and migration by targeting Fyn.
AHO0502 was used in western blot to study the post-transcription regulation of Fyn by miR-25a-3p.
|Ninio-Many L,Grossman H,Shomron N,Chuderland D,Shalgi R||Journal of cell science (126:2867)||2013|
LKB1 represses focal adhesion kinase (FAK) signaling via a FAK-LKB1 complex to regulate FAK site maturation and directional persistence.
AHO0502 was used in western blot to investigate how LKB regulates FAK.
|Kline ER,Shupe J,Gilbert-Ross M,Zhou W,Marcus AI||The Journal of biological chemistry (288:17663)||2013|
Secreted Hsp90 is a novel regulator of the epithelial to mesenchymal transition (EMT) in prostate cancer.
AHO0502 was used in western blot to show a role for eHsp90 in driving epithelial to mesenchymal transition events in prostate cancer.
|Hance MW,Dole K,Gopal U,Bohonowych JE,Jezierska-Drutel A,Neumann CA,Liu H,Garraway IP,Isaacs JS||The Journal of biological chemistry (287:37732)||2012|
Comparative analysis of novel and conventional Hsp90 inhibitors on HIF activity and angiogenic potential in clear cell renal cell carcinoma: implications for clinical evaluation.
AHO0502 was used in western blot to discuss the use of the Hsp90 inhibitor 7-AAG in clinical trials.
|Bohonowych JE,Peng S,Gopal U,Hance MW,Wing SB,Argraves KM,Lundgren K,Isaacs JS||BMC cancer (11:null)||2012|
FAK1, FAK, p125FAK, PPP1R71, FRNK, mKIAA4203, FADK, Fadk, pp125FAK
FADK 1, focal adhesion kinase 1, focal adhesion kinase-related nonkinase, focal ashension kinase 1, pp125FAK, protein-tyrosine kinase 2, FAK-related non-kinase polypeptide, PTK2 protein tyrosine kinase 2, protein phosphatase 1 regulatory subunit 71, protein phosphatase 1, regulatory subunit 71, EC 184.108.40.206, FAK1, Focal adhesion kinase 1, PTK2, Protein- tyrosine kinase 2