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Invitrogen
FAK Polyclonal Antibody
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FIGURE: 1 / 45
FAK Antibody (AHO0502) in WB
CRISPR-Cas9 mediated genome editing ofFAK (as confirmed by next generation sequencing) was achieved by using LentiArray™ Lentiviral sgRNA (Product # A32042, AssayID CRISPR627416_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Fig (a) Western blot analysis of FAK was performed by loading 30 µg of A549 wild type (Lane 1), A549 Cas9 (Lane 2) and A549 Cas9 cells transduced with FAK Lentiviral sgRNA (Lane 3) membrane enriched extracts. The samples were electrophoresed using NuPAGE™ 3 to 8%, Tris-Acetate, 1.0 mm, Mini Protein Gel (Product... View More
Product Details
AHO0502
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to amino acid residues 853-1052 from theC-terminal region of human FAK.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 0.2% BSA
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Focal Adhesion Kinase (FAK) is a 125 kDa non-receptor protein tyrosine kinase that acts as a substrate for Src and is a key element of integrin signaling. FAK plays an important role in cell spreading, differentiation, migration, cell death, and acceleration of the G1 to S phase transition of the cell cycle. FAK has a central catalytic domain and a C-terminal tail that localizes it to focal adhesions, which are sites where cells attach to the extracellular matrix via surface integrin receptors. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin. Adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3, the association of v-Src, Grb2, and Sos with FAK is independent of cell adhesion to fibronectin. In vitro the Grb2 SH2 domain binds directly to tyrosine-phosphorylated FAK, and the binding site has been identified as Tyr925 by site directed mutagenesis. Several transcript variants encoding different isoforms have been found for the FAK gene, but the full-length natures of only three of them have been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: EC 2.7.10.2; FADK 1; FAK-related non-kinase polypeptide; Focal adhesion kinase 1; focal adhesion kinase isoform FAK-Del33; Focal adhesion kinase-related nonkinase; focal ashension kinase 1; FRNK; p125FAK; pp125 PTK2; pp125FAK; PPP1R71; Protein phosphatase 1 regulatory subunit 71; protein phosphatase 1, regulatory subunit 71; Protein-tyrosine kinase 2; PTK2 protein tyrosine kinase 2
Gene Aliases: FADK; FAK; FAK1; FRNK; Kiaa4203; mKIAA4203; p125FAK; pp125FAK; PPP1R71; PTK2
UniProt ID: (Human) Q05397, (Mouse) P34152, (Rat) O35346
Entrez Gene ID: (Human) 5747, (Mouse) 14083, (Rat) 25614
actin binding
protein kinase activity
protein tyrosine kinase activity
non-membrane spanning protein tyrosine kinase activity
signal transducer activity
Ras guanyl-nucleotide exchange factor activity
receptor binding
protein binding
ATP binding
JUN kinase binding
protein kinase binding
SH2 domain binding
nucleotide binding
integrin binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
protein complex binding
identical protein binding
phosphatidylinositol 3-kinase binding
MAPK cascade
microtubule cytoskeleton organization
angiogenesis
blood vessel development
vasculogenesis
neuron migration
regulation of protein phosphorylation
positive regulation of protein phosphorylation
protein phosphorylation
movement of cell or subcellular component
cell adhesion
signal complex assembly
epidermal growth factor receptor signaling pathway
transforming growth factor beta receptor signaling pathway
integrin-mediated signaling pathway
JNK cascade
multicellular organism development
positive regulation of cell proliferation
regulation of cell shape
regulation of epithelial cell migration
positive regulation of phosphatidylinositol 3-kinase signaling
positive regulation of smooth muscle cell migration
phosphorylation
peptidyl-tyrosine phosphorylation
central nervous system neuron axonogenesis
negative regulation of cell-cell adhesion
regulation of cell adhesion
extracellular matrix organization
positive regulation of cell growth
positive regulation of cell migration
negative regulation of cell migration
cellular chloride ion homeostasis
regulation of cell adhesion mediated by integrin
peptidyl-tyrosine autophosphorylation
establishment of nucleus localization
regulation of cell proliferation
negative regulation of apoptotic process
endothelial cell migration
innate immune response
regulation of osteoblast differentiation
positive regulation of cell adhesion
positive regulation of protein kinase activity
positive regulation of vasodilation
negative regulation of organ growth
protein autophosphorylation
ephrin receptor signaling pathway
positive regulation of smooth muscle cell proliferation
positive regulation of phagocytosis
negative regulation of axonogenesis
positive regulation of synaptic transmission
regulation of focal adhesion assembly
positive regulation of protein kinase B signaling
negative regulation of synapse assembly
positive regulation of glial cell proliferation
growth hormone receptor signaling pathway
cellular response to transforming growth factor beta stimulus
regulation of substrate adhesion-dependent cell spreading
positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process
negative regulation of anoikis
placenta development
heart morphogenesis
cellular component disassembly involved in execution phase of apoptosis
axon guidance
embryo development
regulation of endothelial cell migration
establishment of cell polarity
netrin-activated signaling pathway
Fc-gamma receptor signaling pathway involved in phagocytosis
regulation of GTPase activity
positive regulation of GTPase activity
positive regulation of phosphatidylinositol 3-kinase activity
vascular endothelial growth factor receptor signaling pathway
cell motility
regulation of cytoskeleton organization
cytoskeleton organization
response to mechanical stimulus
response to glucose
response to organic substance
response to organonitrogen compound
response to organic cyclic compound
response to estradiol
response to drug
fat cell differentiation
response to arsenic-containing substance
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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