Invitrogen

FAS (CD95) Polyclonal Antibody

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FAS (CD95) Polyclonal Antibody

Product Details

PA5-79235

Applications
Tested Dilution
Publications

Western Blot (WB)

0.1-0.5 µg/mL
-

Immunohistochemistry (Paraffin) (IHC (P))

0.5-1 µg/mL
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived human Fas recombinant protein (Position: Q26-N173).
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 5mg BSA

Contains

0.05mg sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2746351

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: HELA whole cell, JURKAT whole cell, A549 whole cell, SMMC whole cell, K562 whole cell. IHC: Human Lung Cancer Tissue.

Target Information

CD95, also known as Fas or APO-1, is a 46 kDa transmembrane glycoprotein that functions as a cell death receptor in the tumor necrosis factor receptor (TNFR) superfamily. It is expressed by a wide range of hematopoietic and non-hematopoietic cells, including monocytes, neutrophils, lymphocytes, and fibroblasts. CD95 also exists in a soluble form weighing approximately 26 kDa. The interaction of CD95 with its ligand, FasL, induces apoptosis, playing a crucial role in peripheral tolerance and anti-viral immune responses. Upon stimulation, CD95 leads to the aggregation of its intracellular Fas-associated death domains (FADD), formation of the death-inducing signaling complex (DISC), and activation of caspases. This cascade results in apoptosis. CD95 is also involved in activating NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and transduces proliferative signals in normal diploid fibroblasts and T cells. There are at least eight alternatively spliced transcript variants encoding seven distinct isoforms of CD95. Isoforms lacking the transmembrane domain may negatively regulate apoptosis mediated by the full-length isoform. The Fas/Fas ligand system is implicated in several human diseases, including AIDS, hepatitis, and cancer, highlighting its importance in immune regulation and disease pathology.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Apo-1 antigen; APO-1 cell surface antigen; Apo-1 Fas; Apo1 antigen; apoptosis antigen 1; apoptosis signaling receptor FAS; Apoptosis-mediating surface antigen FAS; CD95; CD95 antigen; Fas (TNF receptor superfamily, member 6); Fas AMA; FASLG receptor; OTTHUMP00000020045; OTTHUMP00000020046; OTTHUMP00000020051; OTTHUMP00000059646; TNF receptor superfamily member 6; Tumor necrosis factor receptor superfamily member 6; tumor necrosis factor receptor superfamily, member 6

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Gene Aliases: ALPS1A; APO-1; APT1; CD95; FAS; FAS1; FASTM; TNFRSF6

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UniProt ID: (Human) P25445

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Entrez Gene ID: (Human) 355

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Function(s)
protease binding signal transducer activity receptor activity tumor necrosis factor-activated receptor activity protein binding kinase binding protein complex binding identical protein binding transmembrane signal receptor
Process(es)
ovarian follicle atresia immunoglobulin production renal system process protein complex assembly apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process activation-induced cell death of T cells inflammatory cell apoptotic process inflammatory response immune response signal transduction spermatogenesis brain development aging circadian rhythm extrinsic apoptotic signaling pathway via death domain receptors response to toxic substance gene expression regulation of gene expression B cell mediated immunity telencephalon development dendrite regeneration positive regulation of protein homooligomerization response to lipopolysaccharide tumor necrosis factor-mediated signaling pathway regulation of cell proliferation response to drug ovulation cycle regulation of apoptotic process chordate embryonic development positive regulation of apoptotic process negative regulation of apoptotic process regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of MAPK cascade response to peptide hormone negative thymic T cell selection regulation of lymphocyte differentiation regulation of myeloid cell differentiation response to cycloheximide spleen development negative regulation of B cell activation protein homooligomerization response to glucocorticoid maternal process involved in female pregnancy positive regulation of lymphocyte apoptotic process cellular response to hydrogen peroxide response to growth factor cellular response to phenylalanine cellular response to mechanical stimulus cellular response to cobalt ion cellular response to lithium ion cellular response to glucose stimulus cellular response to interleukin-1 cellular response to estrogen stimulus cellular response to hyperoxia cellular response to hypoxia cellular response to hydrostatic pressure motor neuron apoptotic process apoptotic signaling pathway extrinsic apoptotic signaling pathway extrinsic apoptotic signaling pathway in absence of ligand hepatocyte apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway liver regeneration necroptotic signaling pathway regulation of extrinsic apoptotic signaling pathway via death domain receptors negative regulation of extrinsic apoptotic signaling pathway via death domain receptors response to fluoride positive regulation of extrinsic apoptotic signaling pathway in absence of ligand
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