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FBXO22 Polyclonal Antibody

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Antibody Testing Data (8)

FBXO22 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 8

FBXO22 Antibody (13606-1-AP) in IHC (P)

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 13606-1-AP (FBXO22 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

FBXO22 Antibody in Immunoprecipitation (IP)

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Product Details

13606-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 16 publications 16 publications

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

FBXO22 Fusion Protein Ag4485 (104-403 aa encoded by BC039024)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.17 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody recognizes isoform 1 (45 kDa) and isoform 3 (30-33 kDa).

Immunogen sequence: MADSETFIS LEECRGHKRA RKRTSMETAL ALEKLFPKQC QVLGIVTPGI VVTPMGSGSN RPQEIEIGES GFALLFPQIE GIKIQPFHFI KDPKNLTLER HQLTEVGLLD NPELRVVLVF GYNCCKVGAS NYLQQVVSTF SDMNIILAGG QVDNLSSLTS EKNPLDIDAS GVVGLSFSGH RIQSATVLLN EDVSDEKTAE AAMQRLKAAN IPEHNTIGFM FACVGRGFQY YRAKGNVEAD AFRKFFPSVP LFGFFGNGEI GCDRIVTGNF ILRKCNEVKD DDLFHSYTTI MALIHLGSSK (104-403 aa encoded by BC039024)

Target Information

This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Two transcript variants encoding different isoforms exist for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

High dose isoleucine stabilizes nuclear PTEN to suppress the proliferation of lung cancer.

Discover. Oncology

Wang H,Chen S,Kang W,Ding B,Cui S,Zhou L,Zhang N,Luo H,Wang M,Zhang F,Zhao Z,Guo Z,Wang C,Li L,Wang Z,Chen X,Wang Y

Thu Feb 23 00:00:00 EST 2023

Fbxo22 inhibits metastasis in triple-negative breast cancer through ubiquitin modification of KDM5A and regulation of H3K4me3 demethylation.

Cell biology and toxicology

Li S,He J,Liao X,He Y,Chen R,Chen J,Hu S,Sun J

Tue Aug 01 00:00:00 EDT 2023

Fbxo22 promotes cervical cancer progression via targeting p57^Kip2 for ubiquitination and degradation.

Cell death & disease

Lin M,Zhang J,Bouamar H,Wang Z,Sun LZ,Zhu X

Tue Sep 20 00:00:00 EDT 2022

Fbxo22 promotes cervical cancer progression via targeting p57^Kip2 for ubiquitination and degradation.

Cell death & disease

Lin M,Zhang J,Bouamar H,Wang Z,Sun LZ,Zhu X

Tue Sep 20 00:00:00 EDT 2022

A small-molecule Skp1 inhibitor elicits cell death by p53-dependent mechanism.

iScience

Hussain M,Lu Y,Tariq M,Jiang H,Shu Y,Luo S,Zhu Q,Zhang J,Liu J

Fri Jul 15 00:00:00 EDT 2022

Global identification of phospho-dependent SCF substrates reveals a FBXO22 phosphodegron and an ERK-FBXO22-BAG3 axis in tumorigenesis.

Cell death and differentiation

Liu P,Cong X,Liao S,Jia X,Wang X,Dai W,Zhai L,Zhao L,Ji J,Ni D,Liu Z,Chen Y,Pan L,Liu W,Zhang J,Huang M,Liu B,Tan M

Sat Jan 01 00:00:00 EST 2022

Biotinylation of an acetylenic tricyclic bis(cyanoenone) lowers its potency as an NRF2 activator while creating a novel activity against BACH1.

Free radical biology & medicine

Moreno R,Casares L,Higgins M,Ali KX,Honda T,Wiel C,Sayin VI,Dinkova-Kostova AT,de la Vega L

Sat Oct 01 00:00:00 EDT 2022

BACH1 Expression Is Promoted by Tank Binding Kinase 1 (TBK1) in Pancreatic Cancer Cells to Increase Iron and Reduce the Expression of E-Cadherin.

Antioxidants (Basel, Switzerland)

Liu L,Matsumoto M,Matsui-Watanabe M,Ochiai K,Callens BKK,Nguyen LC,Kozuki Y,Tanaka M,Nishizawa H,Igarashi K

Wed Jul 27 00:00:00 EDT 2022

Pan-Cancer Analyses Reveal Oncogenic Role and Prognostic Value of F-Box Only Protein 22.

Frontiers in oncology

Chen S,Ma S,Yan J,Wang H,Ding B,Guo Z,Ma Y,Chen X,Wang Y

Wed Feb 16 00:00:00 EST 2022

FBXO22 Promotes Growth and Metastasis and Inhibits Autophagy in Epithelial Ovarian Cancers via the MAPK/ERK Pathway.

Frontiers in pharmacology

Li M,Zhao X,Yong H,Shang B,Lou W,Wang Y,Bai J

Sat Dec 25 00:00:00 EST 2021

FBXO22 Promotes Growth and Metastasis and Inhibits Autophagy in Epithelial Ovarian Cancers via the MAPK/ERK Pathway.

Frontiers in pharmacology

Li M,Zhao X,Yong H,Shang B,Lou W,Wang Y,Bai J

Sat Dec 25 00:00:00 EST 2021

FBXO22 mediates polyubiquitination and inactivation of LKB1 to promote lung cancer cell growth.

Cell death & disease

Zhu XN,He P,Zhang L,Yang S,Zhang HL,Zhu D,Liu MD,Yu Y

Wed Jun 19 00:00:00 EDT 2019

FBXO22 mediates polyubiquitination and inactivation of LKB1 to promote lung cancer cell growth.

Cell death & disease

Zhu XN,He P,Zhang L,Yang S,Zhang HL,Zhu D,Liu MD,Yu Y

Wed Jun 19 00:00:00 EDT 2019

Nrf2 Activation Promotes Lung Cancer Metastasis by Inhibiting the Degradation of Bach1.

Cell

Lignitto L,LeBoeuf SE,Homer H,Jiang S,Askenazi M,Karakousi TR,Pass HI,Bhutkar AJ,Tsirigos A,Ueberheide B,Sayin VI,Papagiannakopoulos T,Pagano M

Thu Jul 11 00:00:00 EDT 2019

Nrf2 Activation Promotes Lung Cancer Metastasis by Inhibiting the Degradation of Bach1.

Cell

Lignitto L,LeBoeuf SE,Homer H,Jiang S,Askenazi M,Karakousi TR,Pass HI,Bhutkar AJ,Tsirigos A,Ueberheide B,Sayin VI,Papagiannakopoulos T,Pagano M

Thu Jul 11 00:00:00 EDT 2019

FBXO22 Suppresses Metastasis in Human Renal Cell Carcinoma via Inhibiting MMP-9-Mediated Migration and Invasion and VEGF-Mediated Angiogenesis.

International journal of biological sciences

Guo F,Liu J,Han X,Zhang X,Lin T,Wang Y,Bai J,Han J

Mon Jan 13 00:00:00 EST 2020

FBXO22 Suppresses Metastasis in Human Renal Cell Carcinoma via Inhibiting MMP-9-Mediated Migration and Invasion and VEGF-Mediated Angiogenesis.

International journal of biological sciences

Guo F,Liu J,Han X,Zhang X,Lin T,Wang Y,Bai J,Han J

Mon Jan 13 00:00:00 EST 2020

FBXO22 promotes the development of hepatocellular carcinoma by regulating the ubiquitination and degradation of p21.

Journal of experimental & clinical cancer research : CR

Zhang L,Chen J,Ning D,Liu Q,Wang C,Zhang Z,Chu L,Yu C,Liang HF,Zhang B,Chen X

Tue Feb 26 00:00:00 EST 2019

FBXO22 promotes the development of hepatocellular carcinoma by regulating the ubiquitination and degradation of p21.

Journal of experimental & clinical cancer research : CR

Zhang L,Chen J,Ning D,Liu Q,Wang C,Zhang Z,Chu L,Yu C,Liang HF,Zhang B,Chen X

Tue Feb 26 00:00:00 EST 2019

Phosphorylation of BACH1 switches its function from transcription factor to mitotic chromosome regulator and promotes its interaction with HMMR.

The Biochemical journal

Li J,Shima H,Nishizawa H,Ikeda M,Brydun A,Matsumoto M,Kato H,Saiki Y,Liu L,Watanabe-Matsui M,Iemura K,Tanaka K,Shiraki T,Igarashi K

Thu Mar 15 00:00:00 EDT 2018

Phosphorylation of BACH1 switches its function from transcription factor to mitotic chromosome regulator and promotes its interaction with HMMR.

The Biochemical journal

Li J,Shima H,Nishizawa H,Ikeda M,Brydun A,Matsumoto M,Kato H,Saiki Y,Liu L,Watanabe-Matsui M,Iemura K,Tanaka K,Shiraki T,Igarashi K

Thu Mar 15 00:00:00 EDT 2018

F-Box Protein FBXO22 Mediates Polyubiquitination and Degradation of CD147 to Reverse Cisplatin Resistance of Tumor Cells.

International journal of molecular sciences

Wu B,Liu ZY,Cui J,Yang XM,Jing L,Zhou Y,Chen ZN,Jiang JL

Fri Jan 20 00:00:00 EST 2017

F-Box Protein FBXO22 Mediates Polyubiquitination and Degradation of CD147 to Reverse Cisplatin Resistance of Tumor Cells.

International journal of molecular sciences

Wu B,Liu ZY,Cui J,Yang XM,Jing L,Zhou Y,Chen ZN,Jiang JL

Fri Jan 20 00:00:00 EST 2017

Targeted inhibition of the COP9 signalosome for treatment of cancer.

Nature communications

Schlierf A,Altmann E,Quancard J,Jefferson AB,Assenberg R,Renatus M,Jones M,Hassiepen U,Schaefer M,Kiffe M,Weiss A,Wiesmann C,Sedrani R,Eder J,Martoglio B

Mon Oct 24 00:00:00 EDT 2016

Targeted inhibition of the COP9 signalosome for treatment of cancer.

Nature communications

Schlierf A,Altmann E,Quancard J,Jefferson AB,Assenberg R,Renatus M,Jones M,Hassiepen U,Schaefer M,Kiffe M,Weiss A,Wiesmann C,Sedrani R,Eder J,Martoglio B

Mon Oct 24 00:00:00 EDT 2016

Quantitative proteome analysis of age-related changes in mouse gastrocnemius muscle using mTRAQ.

Proteomics

Hwang CY,Kim K,Choi JY,Bahn YJ,Lee SM,Kim YK,Lee C,Kwon KS

Wed Jan 01 00:00:00 EST 2014

Quantitative proteome analysis of age-related changes in mouse gastrocnemius muscle using mTRAQ.

Proteomics

Hwang CY,Kim K,Choi JY,Bahn YJ,Lee SM,Kim YK,Lee C,Kwon KS

Wed Jan 01 00:00:00 EST 2014

Bioinformatics

Protein Aliases: F-box only protein 22; F-box protein FBX22p44; FIST domain containing 1

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Gene Aliases: FBX22; FBXO22; FISTC1

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UniProt ID: (Human) Q8NEZ5

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Entrez Gene ID: (Human) 26263

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Function(s)

ubiquitin-protein transferase activity

Process(es)

protein polyubiquitination cellular protein modification process ubiquitin-dependent protein catabolic process nucleocytoplasmic transport cellular response to starvation positive regulation of proteasomal ubiquitin-dependent protein catabolic process regulation of skeletal muscle fiber development positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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FBXO22 Polyclonal Antibody

FBXO22 Antibody (13606-1-AP) in IHC (P)

Product Details

13606-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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