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Invitrogen
FER Polyclonal Antibody
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FIGURE: 1 / 1
FER Antibody (PA5-121237) in WB
Western blot analysis of FER in extracts of various cell lines. Samples were incubated with FER Polyclonal antibody (Product # PA5-121237) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-121237
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 200-450 of human FER (NP_005237.2).
Conjugate
Form
Liquid
Concentration
0.73 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive test controls include: HeLa, HT-1080, SW480, LO2, NIH/3T3, Mouse liver, Mouse heart, Rat brain. The target is usually found in the following locations: Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoplasmic side, Membrane, Nucleus, Peripheral membrane protein, cell cortex, cytoskeleton.
Immunogen sequence: YDITLPLLLD SLQKMQEEMI KALKGIFDEY SQITSLVTEE IVNVHKEIQM SVEQIDPSTE YNNFIDVHRT TAAKEQEIEF DTSLLEENEN LQANEIMWNN LTAESLQVML KTLAEELMQT QQMLLNKEEA VLELEKRIEE SSETCEKKSD IVLLLSQKQA LEELKQSVQQ LRCTEAKFSA QKELLEQKVQ ENDGKEPPPV VNYEEDARSV TSMERKERLS KFESIRHSIA GIIRSPKSAL GSSALSDMIS I
Target Information
FER is a member of the FPS/FES family of non-receptor tyrosine kinases. It regulates cell-cell adhesion and mediates signaling from the cell surface to the cytoskeleton via growth factor receptors. FER is found in the cytoplasm and in the nucleus, where it was associated with the chromatin fraction. Its ubiquitous expression and its subcellular location indicate that it may be involved in key regulatory processes. The group IV cytoplasmic protein-tyrosine kinase Fer has been linked to cellular signaling responses to many different stimuli, including growth factors and cytokines. Data suggest a biological role for Fer in regulation of leukocyte recruitment during the innate immune response.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: c-FER; EC 2.7.10.2; Feline encephalitis virus-related kinase FER; fer (fms/fps related) protein kinase, testis specific 2; fer (fps/fes related) tyrosine kinase; Fujinami poultry sarcoma/Feline sarcoma-related protein Fer; p47; p94-FER; phosphoprotein NCP94; protein phosphatase 1, regulatory subunit 74; Proto-oncogene c-Fer; proto-oncogene tyrosine-protein kinase FER; Tyrosine kinase 3; tyrosine protein kinase FLK; Tyrosine-protein kinase Fer; Tyrosine-protein kinase FLK
Gene Aliases: AV082135; C330004K01Rik; FER; Fert; Fert2; Flk; Flk_retired; p94-Fer; PPP1R74; TYK3
UniProt ID: (Human) P16591, (Mouse) P70451, (Rat) P09760
Entrez Gene ID: (Human) 2241, (Mouse) 14158, (Rat) 301737
protein tyrosine kinase activity
non-membrane spanning protein tyrosine kinase activity
epidermal growth factor receptor binding
protein binding
ATP binding
protein phosphatase 1 binding
lipid binding
nucleotide binding
actin binding
protein kinase activity
receptor binding
cytoskeletal protein binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
Rab GTPase binding
protein kinase binding
gamma-catenin binding
cadherin binding
cell adhesion molecule binding
microtubule cytoskeleton organization
mitotic cell cycle
regulation of protein phosphorylation
protein phosphorylation
chemotaxis
cell adhesion
signal transduction
cell proliferation
regulation of lamellipodium assembly
regulation of fibroblast migration
phosphorylation
peptidyl-tyrosine phosphorylation
cytokine-mediated signaling pathway
cell differentiation
positive regulation of cell migration
positive regulation of actin filament polymerization
actin cytoskeleton reorganization
response to lipopolysaccharide
cellular response to insulin stimulus
negative regulation of mast cell activation involved in immune response
substrate adhesion-dependent cell spreading
cellular response to reactive oxygen species
extracellular matrix-cell signaling
cellular response to macrophage colony-stimulating factor stimulus
response to platelet-derived growth factor
insulin receptor signaling pathway via phosphatidylinositol 3-kinase
peptidyl-tyrosine autophosphorylation
Fc-epsilon receptor signaling pathway
Kit signaling pathway
regulation of epidermal growth factor receptor signaling pathway
regulation of cell proliferation
tyrosine phosphorylation of Stat3 protein
regulation of mast cell degranulation
cell-cell adhesion mediated by cadherin
innate immune response
protein autophosphorylation
platelet-derived growth factor receptor signaling pathway
diapedesis
positive regulation of NF-kappaB transcription factor activity
interleukin-6-mediated signaling pathway
epidermal growth factor receptor signaling pathway
cell migration
positive regulation of cell proliferation
intracellular signal transduction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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