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7TM Antibodies
FFA4 Polyclonal Antibody
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FIGURE: 1 / 2
FFA4 Antibody (7TM0127N) in WB
Detection of non-phosphorylated human FFA4 Receptor. Flp-In TREx 293 harboring human FFA4-eYFP induced to express the receptor construct were treated with vehicle or 10 µM TUG-891 for 5 min. Cells were lysed and receptor construct enriched with a GFP-trap, before immunoblotting with anti-non-phospho-FFA4 at a dilution of 1:1,000 (upper panel) or anti-GFP 1:10, 000 to confirm expression and equal loading of the gel (lower panel).
Product Details
7TM0127N
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide presents the carboxyl - terminal tail of human FFA4.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
Dulbecco's PBS, pH 7.4
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Target Information
An EST for GPR120 has been isolated from a human colon cancer library. G-protein Coupled Receptors (GPCRs) comprise one of the largest families of signaling molecules with more than a thousand members currently predicted to exist. All GPCRs share a structural motif consisting of seven membrane-spanning helices, and exist in both active and inactive forms. An array of activating ligands participate in the conformation of GPCRs which leads to signaling via G-proteins and downstream effectors. Ongoing studies have also shown the vast series of reactions which participate in the negative regulation of GPCRs. This "turn-off" activity has tremendous implications for the physiological action of the cell, and continues to drive pharmacological research for new drug candidates. Two blockbuster drugs which have been developed as GPCR-targeted pharmaceuticals are Zyprexa (Eli Lilly) and Claritin (Schering-Plough) which have multi-billion dollar shares of the mental health and allergy markets, respectively.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Free fatty acid receptor 4; G-protein coupled receptor 120; G-protein coupled receptor 129; G-protein coupled receptor GT01; G-protein coupled receptor PGR4; GPCR120; GPR120; Obesity, susceptibility to, on chromosome 10q; Omega-3 fatty acid receptor 1; similar to NPY2R; unnamed protein product
Gene Aliases: BMIQ10; FFAR4; GPR120; GPR129; GT01; O3FAR1; OB10Q; PGR4
UniProt ID: (Human) Q5NUL3
Entrez Gene ID: (Human) 338557
negative regulation of cytokine production
inflammatory response
signal transduction
G-protein coupled receptor signaling pathway
adenylate cyclase-activating G-protein coupled receptor signaling pathway
phospholipase C-activating G-protein coupled receptor signaling pathway
positive regulation of cytosolic calcium ion concentration
regulation of glucose transport
cell differentiation
negative regulation of interleukin-1 beta production
ghrelin secretion
negative regulation of apoptotic process
fat cell differentiation
positive regulation of osteoblast differentiation
hormone secretion
negative regulation of inflammatory response
white fat cell differentiation
brown fat cell differentiation
detection of chemical stimulus involved in sensory perception of taste
positive regulation of glucagon secretion
positive regulation of ERK1 and ERK2 cascade
negative regulation of somatostatin secretion
positive regulation of brown fat cell differentiation
positive regulation of cold-induced thermogenesis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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