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FIBP Antibody (PA5-92973) in WB
Western blot analysis of FIBP in extracts of various cell lines. Samples were incubated with FIBP Polyclonal antibody (Product # PA5-92973) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-92973
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-364 of human FIBP (NP_0042052)
Conjugate
Form
liquid
Concentration
0.8 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2806566
Product Specific Information
Immunogen sequence: MTSELDIFVG NTTLIDEDVY RLWLDGYSVT DAVALRVRSG ILEQTGATAA VLQSDTMDHY RTFHMLERLL HAPPKLLHQL IFQIPPSRQA LLIERYYAFD EAFVREVLGK KLSKGTKKDL DDISTKTGIT LKSCRRQFDN FKRVFKVVEE MRGSLVDNIQ QHFLLSDRLA RDYAAIVFFA NNRFETGKKK LQYLSFGDFA FCAELMIQNW TLGAVDSQMD DMDMDLDKEF LQDLKELKVL VADKDLLDLH KSLVCTALRG KLGVFSEMEA NFKNLSRGLV NVAAKLTHNK DVRDLFVDLV EKFVEPCRSD HWPLSDVRFF LNQYSASVHS LDGFRHQALW DRYMGTLRGC LLRLYHD; Positive Samples: SW620, MCF7, Rat brain; Cellular Location: Endomembrane system, Nucleus, Peripheral membrane protein
Target Information
Acidic fibroblast growth factor is mitogenic for a variety of different cell types and acts by stimulating mitogenesis or inducing morphological changes and differentiation. The FIBP protein is an intracellular protein that binds selectively to acidic fibroblast growth factor (aFGF). It is postulated that FIBP may be involved in the mitogenic action of aFGF. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Acidic fibroblast growth factor intracellular-binding protein; aFGF intracellular-binding protein; FGF intracellular binding protein; FGF-1 intracellular-binding protein
Gene Aliases: FGFIBP; FIBP; FIBP-1
UniProt ID: (Human) O43427
Entrez Gene ID: (Human) 9158, (Rat) 282837
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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