|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues A(19) S P L K N N D E G S L D(31) of mouse FLASH.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500 - 1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-4167 detects the FLASH protein in Human, rat, and mouse samples.
PA1-4167 has been successfully used in Western blot procedures. By Western blot this antibody detects a ~212 kDa protein representing FLASH in NIH3T3 cells.
The PA1-4167 immunogen is a synthetic peptide corresponding to residues A(19) S P L K N N D E G S L D(31) of mouse FLASH.
Maintain at 4°C for 3 months or at -20°C for longer periods. Stable for 1 year. Avoid repeated freeze/thaw cycles.
Fas is a cell-surface receptor molecule that relays apoptotic (cell death) signals into cells. When Fas is activated by binding of its ligand, the proteolytic protein caspase-8 is recruited to a signalling complex known as DISC by binding to a Fas-associated adapter protein. A new protein, FLASH, contains a motif with oligomerizing activity whose sequence is similar to that of the Caenorhabditis elegans protein CED-4, and another domain (DRD domain) that interacts with a death-effector domain in caspase-8 or in the adapter protein. FLASH is likely a component of the DISC signalling complex and is necessary for the activation of caspase-8 in Fas-mediated apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.