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Agrisera
FLS2/Flagellin-sensitive 2 Polyclonal Antibody
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FIGURE: 1 / 2
FLS2/Flagellin-sensitive 2 Antibody (AS12-1857) in WB
Plant material: Arabidopsis thaliana Col-0 (wild type) fls 2 c mutant (described in Nekrasov et al. 2009). Method: Two-week-old seedlings (grown under short day conditions, 8 h light) were collected and frozen in liquid nitrogen. Approximately 100 mg of plant material were extracted in 0.2 mL of homogenization buffer (250 m M sucrose, 50 m M HEPES-KOH p H 7.5,5% glycerol, 0.5% Triton X-100,50 m M Na 4 P 2 O... View More
Product Details
AS12-1857
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated peptide derived from Arabidopsis thaliana FLS2 sequence.
Conjugate
Form
Lyophilized
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles,Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
Target Information
FLS2 encodes a plasma membrane-localized leucine-rich repeat receptor kinase that functions at the cell surface to detect bacterial flagellin-derived epitopes (notably the conserved peptide flg22) and initiate pattern-triggered immunity. The protein is a single-pass transmembrane receptor with a large extracellular ectodomain composed of multiple leucine-rich repeats (commonly described as 28 LRRs) that mediate ligand perception, flanked by conserved cysteine motifs important for proper maturation and receptor competence, and a cytosolic serine/threonine kinase domain required for downstream signaling. Upon flg22 perception, FLS2 rapidly forms an activated receptor complex with the co-receptor kinase BAK1, enabling transphosphorylation-dependent signal propagation that drives early immune outputs such as oxidative burst, defense gene induction, and broad antibacterial resistance. Loss-of-function alleles disrupt flagellin binding and/or signaling, demonstrating that both the extracellular LRR region and intracellular kinase activity are essential for full receptor function in vivo.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AT5G46330; FLAGELLIN-SENSING 2; FLAGELLIN-SENSITIVE 2; MPL12.13; MPL12.8; MPL12_13
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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