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Immunofluorescent analysis of A549 cells using a FOXG1 polyclonal antibody (Product # PA5-26794). A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with a FOXG1 polyclonal antibody (Product # PA5-26794) (1:25, 1 hr at 37°C). Primary antibody was detected with fluor-conjugated donkey anti-rabbit secondary antibody (green) at 1:400 dilution for 50 min at 37°C). Actin filaments have been labeled with dye-conjugated phalloidin (red). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated synthetic peptide between 225-252 amino acids from the central region of human FOXG1|
|Purification||Protein A, Affinity chromatography|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:10-1:50|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with chicken, canine, rat and Xenopus based on sequence homology.
This gene belongs to the forkhead family of transcription factors which is characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in the development of the brain and telencephalon.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
BF1; BF2; brain factor 1; brain factor 2; FHKL3; FKH2; FKHL1; FKHL2; FKHL3; FKHL4; forkhead box protein G1; forkhead-like 1; forkhead-like 2; forkhead-like 3; forkhead-like 4; forkhead-related protein FKHL1; FOXG1A; FOXG1B; FOXG1C; HBF-1; HBF-2; HBF-3; HBF-G2; HBF2; HFK1; HFK2; HFK3; HNF-3/forkhead homolog, brain factor 1; KHL2; oncogene QIN; QIN
2900064B05Rik; BF-1; BF1; BF2; FHKL3; FKH2; FKHL1; FKHL2; FKHL3; FKHL4; FOXG1; FOXG1A; FOXG1B; FOXG1C; HBF-1; HBF-2; HBF-3; HBF-G2; HBF2; Hfh9; Hfhbf1; HFK1; HFK2; HFK3; KHL2; QIN