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Agrisera
FREE1/Protein FREE1 Polyclonal Antibody
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FIGURE: 1 / 1
FREE1/Protein FREE1 Antibody (AS22-4702) in WB
20 µg/well of total protein extracted from Arabidopsis thaliana seedlings. Exact buffer components were: 50 m M Tris-HCl p H 7.4,150 m M Na Cl, 1 m M EDTA, 1% Triton X-100,1 x protease inhibitor cocktail. Proteins were denatured with exact buffer components at 96°C/ 10 min. Samples were separated in the cold on 10% SDS-PAGE and blotted for 1.5 h to nitrocellulose (pore size of 0.22 µm), using: wet transfer in the cold. Blot was blocked with 1% milk for: 1 h/RT with agitation. Blot was incubated in the primary antibody at a dilution of 1:1,... View More
Product Details
AS22-4702
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated peptide derived from Arabidopsis thaliana FREE1 protein sequence.
Conjugate
Form
Lyophilized
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
Target Information
FREE1 encodes a plant-specific FYVE domain protein that localizes predominantly to endosomal compartments associated with phosphatidylinositol-3-phosphate (PI3P)-enriched membranes and can also function in the nucleus, consistent with roles in both membrane trafficking and gene-regulatory pathways. Structurally, FREE1 contains a FYVE zinc-finger domain that mediates PI3P binding, and reported interaction motifs that enable association with ESCRT machinery (including ESCRT-I via Vps23) as well as additional regions implicated in protein-protein interactions that support pathway-specific functions. Biologically, FREE1 is an essential ESCRT component for multivesicular body (MVB) biogenesis and the vacuolar targeting of ubiquitinated cargo, thereby maintaining plasma membrane protein homeostasis and supporting normal plant growth; loss of FREE1 disrupts intraluminal vesicle formation and cargo delivery to the vacuole. Beyond canonical endosomal sorting, FREE1 contributes to autophagic degradation by functionally connecting ESCRT activity to autophagy-related processes, and has been shown to participate in nutrient-responsive signaling by promoting glutamine-dependent TORC1 activation through interaction with TORC1, indicating a broader role as an intracellular sensor/scaffold integrating membrane identity, trafficking, and metabolic regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AT1G20110; FYVE1; PDE330; PIGMENT DEFECTIVE EMBRYO 330; T20H2.10
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