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FUT8 Antibody (PA5-88872) in WB
Western blot analysis of FUT8 in extracts of various cell lines. Samples were incubated with FUT8 Polyclonal antibody (Product # PA5-88872) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-88872
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 376-575 of human FUT8 (NP_8353671)
Conjugate
Form
Liquid
Concentration
0.85 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2805185
Product Specific Information
Immunogen sequence: FHPIEEYMVH VEEHFQLLAR RMQVDKKRVY LATDDPSLLK EAKTKYPNYE FISDNSISWS AGLHNRYTEN SLRGVILDIH FLSQADFLVC TFSSQVCRVA YEIMQTLHPD ASANFHSLDD IYYFGGQNAH NQIAIYAHQP RTADEIPMEP GDIIGVAGNH WDGYSKGVNR KLGRTGLYPS YKVREKIETV KYPTYPEAEK; Positive Samples: A-549, Mouse kidney; Cellular Location: Golgi apparatus, Golgi stack membrane, Single-pass type II membrane protein
Target Information
Catalyzes the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: alpha (1,6) fucosyltransferase; Alpha-(1,6)-fucosyltransferase; alpha-1,6-fucosyltransferase; Alpha1-6FucT; EC 2.4.1.68; Fucosyltransferase 8; fucosyltransferase 8 (alpha (1,6) fucosyltransferase); GDP-fucose--glycoprotein fucosyltransferase; GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1,6-fucosyltransferase; Glycoprotein 6-alpha-L-fucosyltransferase; MGC26465
Gene Aliases: FUT8
UniProt ID: (Human) Q9P2U5, (Mouse) Q9WTS2
Entrez Gene ID: (Human) 2530, (Mouse) 53618
in utero embryonic development
protein N-linked glycosylation
N-glycan processing
transforming growth factor beta receptor signaling pathway
integrin-mediated signaling pathway
respiratory gaseous exchange
oligosaccharide biosynthetic process
regulation of gene expression
cell migration
protein N-linked glycosylation via asparagine
protein glycosylation in Golgi
N-glycan fucosylation
L-fucose catabolic process
receptor metabolic process
GDP-L-fucose metabolic process
regulation of cellular response to oxidative stress
fucosylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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