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Fibromodulin Antibody (PA5-109733) in WB
Western blot analysis of Fibromodulin in extracts of various cell lines. Samples were incubated with Fibromodulin Polyclonal antibody (Product # PA5-109733) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-109733
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 19-376 of human FMOD (NP_002014.2).
Conjugate
Form
Liquid
Concentration
0.69 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2855144
Product Specific Information
Immunogen sequence: QYEDDPHWWF HYLRSQQSTY YDPYDPYPYE TYEPYPYGVD EGPAYTYGSP SPPDPRDCPQ ECDCPPNFPT AMYCDNRNLK YLPFVPSRMK YVYFQNNQIT SIQEGVFDNA TGLLWIALHG NQITSDKVGR KVFSKLRHLE RLYLDHNNLT RMPGPLPRSL RELHLDHNQI SRVPNNALEG LENLTALYLQ HNEIQEVGSS MRGLRSLILL DLSYNHLRKV PDGLPSALEQ LYMEHNNVYT VPDSYFRGAP KLLYVRLSHN SLTNNGLASN TFNSSSLLEL DLSYNQLQKI PPVNTNLENL YLQGNRINEF SISSFCTVVD VVNFSKLQVL RLDGNEIKRS AMPADAPLCL RLASLIEI
Target Information
Fibromodulin is a member of a family of small interstitial proteoglycans, containing a central region composed of leucine-rich repeats with 4 keratan sulfate chains flanked by disulfide-bonded terminal domains. It may participate in the assembly of the extracellular matrix as it interacts with type I and type II collagen fibrils and inhibits fibrillogenesis in vitro. It may also regulate TGF-beta activities by sequestering TGF-beta into the extracellular matrix.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Collagen-binding 59 kDa protein; Fibromodulin; FM; Keratan sulfate proteoglycan fibromodulin; KSPG fibromodulin
Gene Aliases: AI131919; AU041740; FM; FMOD; SLRR2E
UniProt ID: (Human) Q06828, (Mouse) P50608, (Rat) P50609
Entrez Gene ID: (Human) 2331, (Mouse) 14264, (Rat) 64507
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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