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Invitrogen
Fibronectin Polyclonal Antibody
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FIGURE: 1 / 2
Fibronectin Antibody (PA5-99312) in IHC (P)
Immunohistochemistry analysis of paraffin-embedded Fibronectin in human brain tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Fibronectin polyclonal antibody (Product # PA5-99312) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Product Details
PA5-99312
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human FN1(Accession P02751), corresponding to amino acid residues Q2352-E2386.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total Fibronectin.
Target Information
Fibronectin is a disulfide-bonded dimer with a molecular weight range of 230-250 kDa. In the extracellular matrix of several connective tissues and vessels, fibronectin is present as an insoluble protein that is extensively cross-linked by interchain disulfide bonds forming high molecular mass polymers. Fibronectin is most abundant during embryonic development and tissue remodeling. Fibronectin is also present at high concentrations as a soluble plasma protein. Fibronectin is organized as a linear series of repeating modules which form domains for interaction with fibronectin itself, other matrix components (e.g. collagen and heparin) and receptors on cells (e.g. integrins). Fibronectin is present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. Fibronectin has been implicated in carcinoma development in lung cancer. Further, Fibronectin expression is increased especially in non-small cell lung carcinoma. The adhesion of lung carcinoma cells to Fibronectin enhances tumorgenecity and confers resistance to apoptosis induced by standard chemotherapeutic agents. The gene encoding Fibronectin has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some Fibronectin variants has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CIG; Cold insoluble globulin (CIG); Cold-insoluble globulin; DKFZp686F10164; DKFZp686H0342; DKFZp686I1370; DKFZp686O13149; epididymis secretory sperm binding protein; extracellular matrix protein; ferritin L subunit; ferritin L-chain; Fibronectin; FN; lnc-ABCA12-8; Migration stimulating factor (MSF); migration-stimulating factor; unnamed protein product
Gene Aliases: CIG; E330027I09; ED-B; FIBNEC; FINC; FN; Fn-1; FN1; FNZ; GFND; GFND2; LETS; MSF; SMDCF
UniProt ID: (Human) P02751, (Mouse) P11276
Entrez Gene ID: (Human) 2335, (Mouse) 14268, (Rat) 25661
protease binding
receptor binding
integrin binding
extracellular matrix structural constituent
protein binding
collagen binding
heparin binding
peptidase activator activity
identical protein binding
proteoglycan binding
receptor agonist activity
mercury ion binding
extracellular matrix structural protein
angiogenesis
regulation of protein phosphorylation
acute-phase response
cell-substrate junction assembly
cell adhesion
cell-matrix adhesion
calcium-independent cell-matrix adhesion
integrin-mediated signaling pathway
nervous system development
heart development
positive regulation of cell proliferation
regulation of cell shape
response to wounding
positive regulation of gene expression
response to muscle activity
integrin activation
substrate adhesion-dependent cell spreading
endodermal cell differentiation
wound healing
endothelial cell migration
positive regulation of axon extension
positive regulation of fibroblast proliferation
interaction with symbiont
positive regulation of protein kinase B signaling
regulation of ERK1 and ERK2 cascade
negative regulation of transforming growth factor beta production
blood coagulation, fibrin clot formation
negative regulation of monocyte activation
neural crest cell migration involved in autonomic nervous system development
positive regulation of substrate-dependent cell migration, cell attachment to substrate
cell activation
glial cell migration
positive regulation of peptidase activity
peptide cross-linking
extracellular matrix organization
positive regulation of cell migration
negative regulation of apoptotic process
positive regulation of chemotaxis
cellular response to mercury ion
cellular response to interleukin-1
ossification
response to ischemia
response to ozone
cellular response to vascular endothelial growth factor stimulus
cellular response to platelet-derived growth factor stimulus
response to glucocorticoid
cellular response to lipopolysaccharide
cellular response to glucose stimulus
cellular response to prostaglandin E stimulus
cellular response to transforming growth factor beta stimulus
cellular response to BMP stimulus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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