Proteintech

Flotillin 1 Polyclonal Antibody

View all (17) Flotillin 1 antibodies

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Flotillin 1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Hela cells, using FLOT1 antibody 15571-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Flotillin 1 Antibody in Western Blot (WB) — Various lysates were subjected to SDS PAGE followed by western blot with 15571-1-AP (Flotillin 1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Flotillin 1 Antibody in Flow Cytometry (Flow) — 1X10^6 HeLa cells were stained with 0.05ug Flotillin 1 antibody (15571-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488 -Goat anti-Rabbit IGG with dilution 1:100.

Flotillin 1 Antibody in Immunoprecipitation (IP) — IP result of anti-Flotillin 1 (IP:15571-1-AP, 4ug; Detection:15571-1-AP 1:500) with mouse brain tissue lysate 4400ug.

Product Details

15571-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:6,000	View 7 publications 7 publications
Immunohistochemistry (IHC)	Assay-dependent	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	1:10-1:100	-
Flow Cytometry (Flow)	0.05 µg/1x10^6 cells	-
Immunoprecipitation (IP)	0.5-4.0 µg	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Flotillin 1 Fusion Protein Ag7902 (80-427 aa encoded by BC001146) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.33 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MLAAACQMF LGKTEAEIAH IALETLEGHQ RAIMAHMTVE EIYKDRQKFS EQVFKVASSD LVNMGISVVS YTLKDIHDDQ DYLHSLGKAR TAQVQKDARI GEAEAKRDAG IREAKAKQEK VSAQYLSEIE MAKAQRDYEL KKAAYDIEVN TRRAQADLAY QLQVAKTKQQ IEEQRVQVQV VERAQQVAVQ EQEIARREKE LEARVRKPAE AERYKLERLA EAEKSQLIMQ AEAEAASVRM RGEAEAFAIG ARARAEAEQM AKKAEAFQLY QEAAQLDMLL EKLPQVAEEI SGPLTSANKI TLVSSGSGTM GAAKVTGEVL DILTRLPESV ERLTGVSISQ VNHKPLRTA (80-427 aa encoded by BC001146)

Target Information

Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. FLOT1 encodes a caveolae-associated, integral membrane protein. The function of flotillin 1 has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Exosomes Induce Fibroblast Differentiation into Cancer-Associated Fibroblasts through TGFβ Signaling.

Molecular cancer research : MCR

Ringuette Goulet C,Bernard G,Tremblay S,Chabaud S,Bolduc S,Pouliot F

Sun Jul 01 00:00:00 EDT 2018

Identification and characterization of the lamprey Flotillin-1 gene with a role in cell adhesion.

Fish & shellfish immunology

Xu R,Song X,Su P,Pang Y,Li Q

Fri Dec 01 00:00:00 EST 2017

Identification and characterization of the lamprey Flotillin-1 gene with a role in cell adhesion.

Fish & shellfish immunology

Xu R,Song X,Su P,Pang Y,Li Q

Fri Dec 01 00:00:00 EST 2017

Differential protein analysis of serum exosomes post-intravenous immunoglobulin therapy in patients with Kawasaki disease.

Cardiology in the young

Zhang L,Song QF,Jin JJ,Huang P,Wang ZP,Xie XF,Gu XQ,Gao XJ,Jia HL

Wed Nov 01 00:00:00 EDT 2017

Lost region in amyloid precursor protein (APP) through TALEN-mediated genome editing alters mitochondrial morphology.

Scientific reports

Wang Y,Wu F,Pan H,Zheng W,Feng C,Wang Y,Deng Z,Wang L,Luo J,Chen S

Mon Feb 29 00:00:00 EST 2016

TNFα triggers release of extracellular vesicles containing TNFR1 and TRADD, which can modulate TNFα responses of the parental cells.

Archives of biochemistry and biophysics

Sohda M,Misumi Y,Oda K

Tue Dec 01 00:00:00 EST 2015

Flotillin-2 promotes metastasis of nasopharyngeal carcinoma by activating NF-κB and PI3K/Akt3 signaling pathways.

Scientific reports

Liu J,Huang W,Ren C,Wen Q,Liu W,Yang X,Wang L,Zhu B,Zeng L,Feng X,Zhang C,Chen H,Jia W,Zhang L,Xia X,Chen Y

Fri Jul 24 00:00:00 EDT 2015

Flotillin-2 promotes metastasis of nasopharyngeal carcinoma by activating NF-κB and PI3K/Akt3 signaling pathways.

Scientific reports

Liu J,Huang W,Ren C,Wen Q,Liu W,Yang X,Wang L,Zhu B,Zeng L,Feng X,Zhang C,Chen H,Jia W,Zhang L,Xia X,Chen Y

Fri Jul 24 00:00:00 EDT 2015

Translocation of mixed lineage kinase domain-like protein to plasma membrane leads to necrotic cell death.

Cell research

Chen X,Li W,Ren J,Huang D,He WT,Song Y,Yang C,Li W,Zheng X,Chen P,Han J

Wed Jan 01 00:00:00 EST 2014

Bioinformatics

Protein Aliases: DADB-118P11.2; Flotillin-1; integral membrane component of caveolae; OTTHUMP00000164855; REG-2; Reggie-2

Gene Aliases: FLOT1; Rareg; Reg2; reggie-2

UniProt ID: (Human) O75955, (Mouse) O08917, (Rat) Q9Z1E1

Entrez Gene ID: (Human) 10211, (Mouse) 14251, (Rat) 64665

membrane raft assembly positive regulation of cytokine production positive regulation of protein phosphorylation regulation of receptor internalization endocytosis axonogenesis axon guidance extracellular matrix disassembly positive regulation of protein binding positive regulation of interferon-beta production dsRNA transport positive regulation of heterotypic cell-cell adhesion positive regulation of toll-like receptor 3 signaling pathway response to endoplasmic reticulum stress regulation of Rho protein signal transduction plasma membrane raft assembly positive regulation of endocytosis positive regulation of skeletal muscle tissue development protein homooligomerization positive regulation of cell adhesion molecule production protein kinase C signaling cellular response to exogenous dsRNA protein localization to plasma membrane establishment of protein localization to plasma membrane positive regulation of myoblast fusion positive regulation of cell junction assembly protein localization to membrane raft positive regulation of cell-cell adhesion mediated by cadherin positive regulation of synaptic transmission, dopaminergic regulation of toll-like receptor 4 signaling pathway

Process(es)

protease binding protein binding ionotropic glutamate receptor binding protein heterodimerization activity