Invitrogen

Flotillin 1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

1 Published Figure

1 Reference

View all (17) Flotillin 1 antibodies

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Flotillin 1 Antibody in Western Blot (WB) — Western blot was performed using Anti- Flotillin 1 Polyclonal Antibody (Product # PA5-19713) and a 47kDa band corresponding to Flotillin 1 was observed across all the cell lines and tissue lysates tested except THP1 which is reported negative for Flotillin 1 expression. An uncharacterized band was observed at ~32KDa across all the samples and ~60KDa in THP-1 sample alone. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), HEK-293 (Lane 2), MCF7 (Lane 3), MDA-MB-231 (Lane 4), HeLa (Lane 5), A549 (Lane 6), THP-1 (Lane 7), tissue extracts of Mouse Lung (Lane 8), Mouse Brain (Lane 9) and Mouse Heart (Lane 10) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blots were probed with the primary antibody (1µg/mL) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)..

Flotillin 1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines and tissues owing to their inherent genetic constitution. Relative expression of Flotillin 1 was observed in most of the cell lines and tissue extracts in comparison to THP-1 using Flotillin 1 Polyclonal Antibody (Product # PA5-19713) in western blot. {RE}

Product Details

PA5-19713

Applications	Tested Dilution	Publications
Western Blot (WB)	1 µg/mL	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	1:200	-

Product Specifications
Species Reactivity	Human, Mouse
Published species	Not Applicable
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Flotillin 1. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.9 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.4, with 1% BSA
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_10981198

Product Specific Information

For Western Blot, this antibody has non-specific bands at 35 kDa.

Heat mediated antigen retrieval recommended prior to tissue staining.

This antibody is predicted to react with many species based on sequence homology.

Store antibody at 4°C for 1-2 weeks. For long-term storage, store at -20°C.

Target Information

Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. FLOT1 encodes a caveolae-associated, integral membrane protein. The function of flotillin 1 has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The conserved metalloprotease invadolysin is present in invertebrate haemolymph and vertebrate blood.

Biology open

Abhinav K,Feng L,Morrison E,Jung Y,Dear J,Takahashi S,Heck MMS

PA5-19713 was used in Western Blotting to show that the protease motif of invadolysin is important for its role in lipid accumulation, but not in glycogen accumulation.

Mon Nov 04 00:00:00 EST 2019

Bioinformatics

Protein Aliases: DADB-118P11.2; Flotillin-1; integral membrane component of caveolae; OTTHUMP00000164855

Gene Aliases: FLOT1; reggie-2

UniProt ID: (Human) O75955, (Mouse) O08917

Entrez Gene ID: (Human) 10211, (Mouse) 14251

Function(s)

Process(es)

protease binding protein binding ionotropic glutamate receptor binding protein heterodimerization activity

Flotillin 1 Polyclonal Antibody

Product Details

PA5-19713

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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