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Invitrogen
Fumarase Polyclonal Antibody
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FIGURE: 1 / 1
Fumarase Antibody (PA5-80633) in WB
Western Blot using Fumarase Polyclonal Antibody (Product # PA5-80633) at 1:500 dilution. Lane A: A549 Whole Cell Lysate, Lane B: Hela Whole Cell Lysate. Lysates/proteins at 30 μg per lane. Secondary Goat Anti-Rabbit IgG H&L (DyLight™ 800) at 1:10,000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 55 kDa. Observed band size: 48 kDa.
Product Details
PA5-80633
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human Fumarate Hydratase/FH protein (Ala45-Lys510)
Conjugate
Form
Liquid
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Human Fumarate Hydratase/FH.
Target Information
The protein encoded by this gene is an enzymatic component of the tricarboxylic acid cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: epididymis secretory sperm binding protein; Fumarase; fumarase precursor (EC 4.2.1.2); Fumarate hydratase, mitochondrial; unnamed protein product
Gene Aliases: FH; FMRD; HLRCC; HsFH; LRCC; MCL; MCUL1
UniProt ID: (Human) P07954
Entrez Gene ID: (Human) 2271
urea cycle
regulation of arginine metabolic process
tricarboxylic acid cycle
fumarate metabolic process
malate metabolic process
DNA repair
arginine metabolic process
cellular response to DNA damage stimulus
homeostasis of number of cells within a tissue
positive regulation of cold-induced thermogenesis
positive regulation of double-strand break repair via nonhomologous end joining
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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