Proteintech

G3BP1 Polyclonal Antibody

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G3BP1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol ) fixed HeLa cells using 13057-2-AP (G3BP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

G3BP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 13057-2-AP (G3BP1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

G3BP1 Antibody in Western Blot (WB) — Rat brain tissue were subjected to SDS PAGE followed by western blot with 13057-2-AP (G3BP1 Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

G3BP1 Antibody in Flow Cytometry (Flow) — 1X10^6 HeLa cells were stained with 0.2ug G3BP1 antibody (13057-2-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

G3BP1 Antibody in Immunoprecipitation (IP) — IP result of anti-G3BP1 (IP:13057-2-AP, 3ug; Detection:13057-2-AP 1:1000) with HEK-293 cells lysate 2000ug.

Product Details

13057-2-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:5,000-1:50,000	View 10 publications 10 publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:50-1:500	View 21 publications 21 publications
Flow Cytometry (Flow)	0.2 µg/1x10^6 cells	-
Immunoprecipitation (IP)	0.5-4.0 µg	View 1 publication 1 publication
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	G3BP1 Fusion Protein Ag3728 (167-466 aa encoded by BC006997) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.25 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: PDDSGTFYD QAVVSNDMEE HLEEPVAEPE PDPEPEPEQE PVSEIQEEKP EPVLEETAPE DAQKSSSPAP ADIAQTVQED LRTFSWASVT SKNLPPSGAV PVTGIPPHVV KVPASQPRPE SKPESQIPPQ RPQRDQRVRE QRINIPPQRG PRPIREAGEQ GDIEPRRMVR HPDSHQLFIG NLPHEVDKSE LKDFFQSYGN VVELRINSGG KLPNFGFVVF DDSEPVQKVL SNRPIMFRGE VRLNVEEKKT RAAREGDRRD NRLRGPGGPR GGLGGGMRGP PRGGMVQKPG FGVGRGLAPR Q (167-466 aa encoded by BC006997)

Target Information

This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ATP-dependent DNA helicase VIII; DNA helicase VIII; G3BP-1; GAP binding protein; GAP SH3 binding protein; GAP SH3 domain-binding protein 1; GTPase activating protein (SH3 domain) binding protein 1; hDH VIII; HDH-VIII; MGC111040; Ras GTPase-activating protein-binding protein 1; Ras-GTPase-activating protein SH3-domain binding protein 1; Ras-GTPase-activating protein SH3-domain-binding protein; RasGAP-associated endoribonuclease G3BP

Gene Aliases: AI849976; B430204O07; C87777; G3BP; G3BP1; HDH-VIII; mKIAA4115; RGD1310666

UniProt ID: (Human) Q13283, (Mouse) P97855

Entrez Gene ID: (Human) 10146, (Rat) 171092, (Mouse) 27041

Molecular Function: RNA metabolism protein nucleic acid metabolism protein