Proteintech

G3BP1 Polyclonal Antibody

Product Image Gallery

G3BP1 Antibody in Western Blot (WB) — HepG2 cells were subjected to SDS PAGE followed by western blot with 13057-2-AP (G3BP1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

G3BP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 13057-2-AP (G3BP1 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

G3BP1 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 13057-2-AP (G3BP1 antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

G3BP1 Antibody in Flow Cytometry (Flow) — 1X10^6 HeLa cells were stained with 0.2ug G3BP1 antibody (13057-2-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

G3BP1 Antibody in Immunoprecipitation (IP) — IP result of anti-G3BP1 (IP:13057-2-AP, 3ug; Detection:13057-2-AP 1:1000) with HEK-293 cells lysate 2000ug.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5000-1:50,000

View 10 publications 10 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC)

1:50-1:500

Immunofluorescence (IF)

1:50-1:500

View 22 publications 22 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

G3BP1 Fusion Protein Ag3728

Conjugate

Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Target Information

This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ATP-dependent DNA helicase VIII; DNA helicase VIII; G3BP-1; GAP binding protein; GAP SH3 binding protein; GAP SH3 domain-binding protein 1; GTPase activating protein (SH3 domain) binding protein 1; hDH VIII; HDH-VIII; MGC111040; Ras GTPase-activating protein-binding protein 1; Ras-GTPase-activating protein SH3-domain binding protein 1; Ras-GTPase-activating protein SH3-domain-binding protein; RasGAP-associated endoribonuclease G3BP

Gene Aliases: AI849976; B430204O07; C87777; G3BP; G3BP1; HDH-VIII; mKIAA4115; RGD1310666

UniProt ID: (Human) Q13283, (Mouse) P97855

Entrez Gene ID: (Human) 10146, (Rat) 171092, (Mouse) 27041

Molecular Function: RNA binding protein nucleic acid binding signaling molecule