Invitrogen

G3BP1 Polyclonal Antibody

Product Image Gallery

G3BP1 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of G3BP1 in U-251 MG cells using a G3BP1 polyclonal antibody (Product # PA5-82129). The analysis shows localization to cytosol.

G3BP1 Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of G3BP1 in human testis using a G3BP1 polyclonal antibody (Product # PA5-82129). The analysis shows positivity in cells in seminiferous ducts.

G3BP1 Antibody in Western Blot (WB) — Western blot analysis of G3BP1 by a G3BP1 polyclonal antibody (Product # PA5-82129). Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-G3BP1.

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

1-4 µg/mL

Immunofluorescence (IF)

1-4 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Western Blot (WB)

0.4 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human G3BP1

Conjugate

Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Product Specific Information

Immunogen sequence: FRYQDEVFGG FVTEPQEESE EEVEEPEERQ QTPEVVPDDS GTFYDQAVVS NDMEEHLEEP VAEPEPDPEP EPEQEPVSEI QEEKPEPVLE ETAP

Target Information

This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ATP-dependent DNA helicase VIII; DNA helicase VIII; G3BP; G3BP-1; GAP binding protein; GAP SH3 binding protein; GAP SH3 domain-binding protein 1; GTPase activating protein (SH3 domain) binding protein 1; hDH VIII; HDH-VIII; Ras GTPase-activating protein-binding protein 1; Ras-GTPase-activating protein SH3-domain binding protein 1; Ras-GTPase-activating protein SH3-domain-binding protein; RasGAP-associated endoribonuclease G3BP

Gene Aliases: AI849976; B430204O07; C87777; G3BP; G3BP1; HDH-VIII; mKIAA4115; RGD1310666

UniProt ID: (Human) Q13283, (Mouse) P97855

Entrez Gene ID: (Human) 10146, (Rat) 171092, (Mouse) 27041

Molecular Function: RNA binding protein nucleic acid binding signaling molecule