Invitrogen

G3BP1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

View all (27) G3BP1 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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G3BP1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of G3BP1 in U-251 MG cells using a G3BP1 polyclonal antibody (Product # PA5-82129). The analysis shows localization to cytosol.

G3BP1 Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of G3BP1 in human testis using a G3BP1 polyclonal antibody (Product # PA5-82129). The analysis shows positivity in cells in seminiferous ducts.

G3BP1 Antibody in Western Blot (WB) — Knockout of G3BP1 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR954538_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Western blot analysis of G3BP1 was performed by loading 30 µg of A-431 wild type (Lane 1), A-431 Cas9 (Lane 2) andA-431 G3BP1 KO (Lane 3) whole cell extracts. The samples were electrophoresed using NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with Anti-G3BP1 Polyclonal Antibody (Product # PA5-82129, 1:250 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:5000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). Loss of signal upon CRISPR mediated knockout (KO) using the LentiArray™ CRISPR product line confirms that antibody is specific to G3BP1.

G3BP1 Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. A loss of signal was observed for target protein inG3BP1 KO cell line compared to control cell line using Anti-G3BP1 Polyclonal Antibody (Product # PA5-82129).

Product Details

PA5-82129

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human G3BP1

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Anitgen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2789290

Product Specific Information

Immunogen sequence: FRYQDEVFGG FVTEPQEESE EEVEEPEERQ QTPEVVPDDS GTFYDQAVVS NDMEEHLEEP VAEPEPDPEP EPEQEPVSEI QEEKPEPVLE ETAP

Target Information

This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ATP-dependent DNA helicase VIII; DNA helicase VIII; G3BP-1; GAP binding protein; GAP SH3 binding protein; GAP SH3 domain-binding protein 1; GTPase activating protein (SH3 domain) binding protein 1; hDH VIII; HDH-VIII; MGC111040; Ras GTPase-activating protein-binding protein 1; Ras-GTPase-activating protein SH3-domain binding protein 1; Ras-GTPase-activating protein SH3-domain-binding protein; RasGAP-associated endoribonuclease G3BP

Gene Aliases: AI849976; B430204O07; C87777; G3BP; G3BP1; HDH-VIII; mKIAA4115; RGD1310666

UniProt ID: (Human) Q13283, (Mouse) P97855

Entrez Gene ID: (Human) 10146, (Rat) 171092, (Mouse) 27041

Molecular Function: RNA metabolism protein nucleic acid metabolism protein