Invitrogen

G3BP1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

View all (22) G3BP1 antibodies

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G3BP1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of G3BP1 in U-251 MG cells using a G3BP1 polyclonal antibody (Product # PA5-82129). The analysis shows localization to cytosol.

G3BP1 Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of G3BP1 in human testis using a G3BP1 polyclonal antibody (Product # PA5-82129). The analysis shows positivity in cells in seminiferous ducts.

G3BP1 Antibody in Western Blot (WB) — Western blot analysis of G3BP1 was performed by loading whole cell lysate in 1X LDS sample buffer with 2-ME from 5 x 10^5 mouse transformed pre-B cells transduced with or without guide RNA targeting G3bp1 (gG3bp1). Samples were loaded onto a 4-12 % Bis-Tris polyacrylamide gel (Product # NP0336BOX). Proteins were transferred to nitrocellulose membrane with wet/tank transfer. Membrane was blocked in 5% milk/TBST. The membrane was probe with a polyclonal anti-G3BP1 antibody (Product # PA5-82129) at a dilution of 1:1,000 in 2% milk/TBST at 4°C overnight, followed by a secondary antibody HRP-anti-mouse at a dilution of 1:5,000 at room temperature for 1 hour. Chemiluminescent detection was performed. Data courtesy of Antibody Data Exchange Program.

G3BP1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. A-431 cells were transfected with, control siRNA, G3BP1 siRNA probe #1, and G3BP1 siRNA probe #2 and a decrease in signal intensity was observed in Western Blot application using anti-G3BP1 Polyclonal Antibody (Product # PA5-82129) used at a dilution of 0.4 µg/ml. Anti-G3BP1 was used as a loading control. {KD}

Product Details

PA5-82129

Applications	Tested Dilution	Publications
Western Blot (WB)	0.04-0.4 µg/mL	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	0.25-2 µg/mL	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human G3BP1 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.1 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2789290

Product Specific Information

Immunogen sequence: FRYQDEVFGG FVTEPQEESE EEVEEPEERQ QTPEVVPDDS GTFYDQAVVS NDMEEHLEEP VAEPEPDPEP EPEQEPVSEI QEEKPEPVLE ETAP

Target Information

This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ATP-dependent DNA helicase VIII; DNA helicase VIII; G3BP-1; GAP binding protein; GAP SH3 binding protein; GAP SH3 domain-binding protein 1; GTPase activating protein (SH3 domain) binding protein 1; hDH VIII; HDH-VIII; MGC111040; Ras GTPase-activating protein-binding protein 1; Ras-GTPase-activating protein SH3-domain binding protein 1; Ras-GTPase-activating protein SH3-domain-binding protein; RasGAP-associated endoribonuclease G3BP

Gene Aliases: AI849976; B430204O07; C87777; G3BP; G3BP1; HDH-VIII; mKIAA4115; RGD1310666

UniProt ID: (Human) Q13283, (Mouse) P97855

Entrez Gene ID: (Human) 10146, (Rat) 171092, (Mouse) 27041

Molecular Function: RNA metabolism protein nucleic acid metabolism protein