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Immunofluorescence analysis of GABA neurons using anti-GABA antibody (PA5-32241). GABAergic precursor cells derived from H9 embryonic stem cells were differentiated for 7 days and stained with antibodies against GABA (Cat. No. PA5-32241 at 1:5000) followed by Alexa Fluor 488 goat anti-rabbit (Cat. No. A21206, green) and neuronal marker beta III tubulin (Cat. No. 480011 at 1:1000) followed by by Alexa Fluor 594 goat-anti-mouse (Cat. No. A11005, red). Nuclear DNA was stained with DAPI (blue).
|Tested species reactivity||Chem|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GABA conjugated to KLH|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Dot blot (DB)||1:10,000-1:90,000|
|Immunohistochemistry (Frozen) (IHC (F))||Assay-dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:400|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This chemical acts as a neurotransmitter in the central nervous system. It reduces neuronal excitation and regulates muscle tone.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
gamma-Aminobutyric acid; y-Aminobutyric acid