Immunofluorescence analysis of GABA neurons using anti-GABA antibody (PA5-32241). GABAergic precursor cells derived from H9 embryonic stem cells were differentiated for 7 days and stained with antibodies against GABA (Cat. No. PA5-32241 at 1:5000) followed by Alexa Fluor 488 goat anti-rabbit (Cat. No. A21206, green) and neuronal marker beta III tubulin (Cat. No. 480011 at 1:1000) followed by by Alexa Fluor 594 goat-anti-mouse (Cat. No. A11005, red). Nuclear DNA was stained with DAPI (blue).
|Tested species reactivity||Chemical|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GABA conjugated to KLH|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Dot blot (DB)||1:10,000-1:90,000|
|Immunohistochemistry (Frozen) (IHC (F))||Assay-dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:400|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This chemical acts as a neurotransmitter in the central nervous system. It reduces neuronal excitation and regulates muscle tone.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.