33-8600 detects the Gal4 DNA Binding Domain by western blot, immunoprecipitation and immunofluorescence corresponding to amino acids 1-147 of the GAL4 DNA binding region.
GAL4 is a transcriptional activator that binds lactose and a related range of sugars. Specifically, GAL4 is a positive regulator for the gene expression of galactose-genes that include GAL1 and GAL2 which code for enzymes that convert galactose to glucose. The DNA binding domain of GAL4 is contained in the first 74 amino acids and contains a "zinc finger" sequence motif. However, amino acids 1-147 have been shown to bind to DNA in vitro. While GAL4 (1-74) bind DNA specifically, it is with a lower affinity than the GAL4 (1-147). GAL4 (1-147) binds to a specific 17 base pair nucleotide sequence, but alone is insufficient to activate transcription. Either of the two acidic activating regions, activating region I (amino acids 148-238) or activating region II (amino acids 768-881), must be joined to activate transcription. Research has shown that GAL4 may activate the mouse mammary tumor virus.
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Protein Aliases: antigen NY-CO-27; gal-4; GAL4; galactose-responsive transcription factor GAL4; galectin 4; galectin-4; lactose-binding lectin 4; Regulatory protein GAL4
Gene Aliases: GAL4; GAL81; YPL248C
UniProt ID: (Baker's yeast) P04386
Entrez Gene ID: (Baker's yeast) 855828