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Invitrogen
GATA2 Polyclonal Antibody
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FIGURE: 1 / 9
GATA2 Antibody (PA1-100) in ICC/IF
Immunofluorescent analysis of GATA2 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were then blocked with 5% normal goat serum (Product # 31873) for 15 minutes at room temperature. Cells were then probed with a rabbit polyclonal antibody recognizing GATA2 (Product # PA1-100) at a dilution of 1:200 for at least 1 hour at 37°C. Cells were then washed with PBS and incubated with DyLight 488 goat-anti-rabbit secondary antibody at a dilution of 1:200 for 30 ... View More
Product Details
PA1-100
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
N-terminal truncated human recombinant protein
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS with 1mg/mL BSA, 30% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-100 specifically detects GATA2 and does not cross-react with GATA1 or GATA3.
Target Information
GATA2 (GATA binding protein 2) belongs to the GATA family of transcription factors. GATA proteins play a very important role in signaling pathways controlling proliferation and differentiation of erythroid progenitor cells. There are 6 transcription factors in the GATA family: GATA1 to GATA6. GATA2 is a transcriptional activator regulating endothelin-1 gene expression in endothelial cells. It also appears to be involved in differentiation of other tissue types (uro-genital and nervous) during embryogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Endothelial transcription factor GATA-2; FLJ45948; GATA-binding protein 2; MGC2306
Gene Aliases: DCML; Gata-2; GATA2; IMD21; MONOMAC; NFE1B
UniProt ID: (Human) P23769, (Mouse) O09100
Entrez Gene ID: (Human) 2624, (Mouse) 14461
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II transcription factor binding
enhancer sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
transcription factor binding
zinc ion binding
C2H2 zinc finger domain binding
DNA binding
sequence-specific DNA binding
metal ion binding
DNA-binding transcription factor
negative regulation of transcription from RNA polymerase II promoter
urogenital system development
cell fate determination
neuron migration
embryonic placenta development
transcription, DNA-templated
transcription from RNA polymerase II promoter
phagocytosis
positive regulation of cytosolic calcium ion concentration
heart development
blood coagulation
organ morphogenesis
tissue development
regulation of primitive erythrocyte differentiation
ventral spinal cord interneuron differentiation
cell differentiation in hindbrain
commitment of neuronal cell to specific neuron type in forebrain
central nervous system neuron development
pituitary gland development
response to lipid
somatic stem cell population maintenance
regulation of histone acetylation
eosinophil fate commitment
inner ear morphogenesis
mast cell degranulation
negative regulation of fat cell differentiation
positive regulation of erythrocyte differentiation
negative regulation of macrophage differentiation
positive regulation of megakaryocyte differentiation
positive regulation of neuron differentiation
negative regulation of Notch signaling pathway
positive regulation of angiogenesis
positive regulation of transcription from RNA polymerase II promoter
cell maturation
digestive tract development
homeostasis of number of cells within a tissue
positive regulation of phagocytosis
positive regulation of phagocytosis, engulfment
definitive hemopoiesis
semicircular canal development
negative regulation of fat cell proliferation
GABAergic neuron differentiation
negative regulation of neural precursor cell proliferation
regulation of forebrain neuron differentiation
regulation of transcription, DNA-templated
hemopoiesis
cell differentiation
neuron differentiation
negative regulation of myeloid cell differentiation
neuron fate commitment
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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