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GBF1 Antibody (PA5-27751) in IHC (P)
GBF1 Polyclonal Antibody detects GBF1 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded colon carcinoma. GBF1 Polyclonal Antibody (Product # PA5-27751) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Product Details
PA5-27751
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 193 of Human GBF1
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID
AB_2545227
Product Specific Information
Recommended positive controls: A549, HeLa, HepG2.
Predicted reactivity: Mouse (96%), Rat (96%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene encodes a member of the Sec7 domain family. The encoded protein is a guanine nucleotide exchange factor that regulates the recruitment of proteins to membranes by mediating GDP to GTP exchange. The encoded protein is localized to the Golgi apparatus and plays a role in vesicular trafficking by activating ADP ribosylation factor 1. The encoded protein has also been identified as an important host factor for viral replication. Multiple transcript variants have been observed for this gene.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BFA-resistant GEF 1; Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1
Gene Aliases: ARF1GEF; GBF1; KIAA0248
UniProt ID: (Human) Q92538
Entrez Gene ID: (Human) 8729
Molecular Function:
guanyl-nucleotide exchange factor
cell activation involved in immune response
ER to Golgi vesicle-mediated transport
retrograde vesicle-mediated transport, Golgi to ER
post-Golgi vesicle-mediated transport
Golgi to endosome transport
Golgi organization
regulation of mitotic cell cycle
protein transport
viral process
neutrophil chemotaxis
regulation of ARF protein signal transduction
protein localization to Golgi apparatus
retrograde transport, endosome to Golgi
positive regulation of GTPase activity
COPI coating of Golgi vesicle
establishment of monopolar cell polarity
protein localization to endoplasmic reticulum exit site
Golgi disassembly
endoplasmic reticulum-Golgi intermediate compartment organization
reactive oxygen species biosynthetic process
protein localization to endoplasmic reticulum tubular network
regulation of protein localization to cell surface
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