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Invitrogen
GBL Polyclonal Antibody
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FIGURE: 1 / 3
GBL Antibody (PA5-88005) in WB
Western blot analysis of GBL in extracts of various cell lines. Samples were incubated with GBL Polyclonal antibody (Product # PA5-88005) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Product Details
PA5-88005
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-326 of human MLST8 (NP_0011861031)
Conjugate
Form
Liquid
Concentration
2.13 mg/mL
Amount
213 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MNTSPGTVGS DPVILATAGY DHTVRFWQAH SGICTRTVQH QDSQVNALEV TPDRSMIAAA GYQHIRMYDL NSNNPNPIIS YDGVNKNIAS VGFHEDGRWM YTGGEDCTAR IWDLRSRNLQ CQRIFQVNAP INCVCLHPNQ AELIVGDQSG AIHIWDLKTD HNEQLIPEPE VSITSAHIDP DASYMAAVNS TGNCYVWNLT GGIGDEVTQL IPKTKIPAHT RYALQCRFSP DSTLLATCSA DQTCKIWRTS NFSLMTELSI KSGNPGESSR GWMWGCAFSG DSQYIVTASS DNLARLWCVE TGEIKREYGG HQKAVVCLAF NDSVLG; Positive Samples: 293T, HeLa, K-562, 22Rv1, Mouse brain, Mouse kidney, Mouse thymus, Rat brain, Rat testis; Cellular Location: Cytoplasm
Target Information
G-protein beta subunit like (GBL protein) is a novel member of WD-40 protein family. It is a highly conserved cytosolic protein consisting of seven WD-40 repeating motifs. LST8 is a functional component of mTOR signaling complex (TORC1) and positively up-regulates the mTOR pathway. It interacts with the kinase domain of mTOR and stabilizes its interaction with raptor. LST8 participates in regulating cell growth and also in nutrient and growth factor-mediated mTOR signaling to S6K1. Reports suggest up-regulated expression of LST8 due to insulin indicating a possible role of LST8 in intracellular events related to insulin-receptor activation including insulin-stimulated glucose transport and glycogen synthesis. Northern Blot analysis detected a ubiquitous expression of LST8 with highest expression in brain and testis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: G beta 6; G beta-like protein; guanine nucleotide binding protein; G beta-like protein; G protein beta subunit-like; Gable; GBL protein; Mammalian lethal with SEC13 protein 8; mLST8; mLST8 / mLST8; Protein GbetaL; Target of rapamycin complex subunit LST8; TORC subunit gable; TORC subunit LST8; TORC subunit WAT1; transducin (beta)-like 4; unnamed protein product
Gene Aliases: 0610033N12Rik; AA409454; AI505104; AI851821; GbetaL; GBL; LST8; MLST8; POP3; WAT1
UniProt ID: (Human) Q9BVC4, (Mouse) Q9DCJ1, (Rat) Q9Z2K5
Entrez Gene ID: (Human) 64223, (Mouse) 56716, (Rat) 64226
cellular response to DNA damage stimulus
cytoskeleton organization
negative regulation of autophagy
positive regulation of cell growth
positive regulation of actin filament polymerization
cellular response to nutrient levels
TOR signaling
positive regulation of TOR signaling
regulation of actin cytoskeleton organization
TORC1 signaling
TORC2 signaling
negative regulation of apoptotic process
protein kinase B signaling
positive regulation of glycolytic process
positive regulation of lipid biosynthetic process
positive regulation of protein kinase B signaling
cellular response to hypoxia
cellular response to osmotic stress
positive regulation of pentose-phosphate shunt
regulation of GTPase activity
positive regulation of peptidyl-tyrosine phosphorylation
biological_process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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