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Invitrogen
GBP1 Polyclonal Antibody
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FIGURE: 1 / 1
GBP1 Antibody (PA5-93280) in WB
Western blot analysis of GBP1 in extracts of 5637 (HTB-9) cells. Samples were incubated with GBP1 Polyclonal antibody (Product # PA5-93280) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-93280
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 383-592 of human GBP1 (NP_0020442)
Conjugate
Form
Liquid
Concentration
0.97 mg/mL
Amount
97 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: ELAAQLEKKR DDFCKQNQEA SSDRCSALLQ VIFSPLEEEV KAGIYSKPGG YRLFVQKLQD LKKKYYEEPR KGIQAEEILQ TYLKSKESMT DAILQTDQTL TEKEKEIEVE RVKAESAQAS AKMLQEMQRK NEQMMEQKER SYQEHLKQLT EKMENDRVQL LKEQERTLAL KLQEQEQLLK EGFQKESRIM KNEIQDLQTK MRRRKACTIS; Positive Samples: 5637(HTB-9); Cellular Location: Cell membrane, Cytoplasm, Cytoplasmic side, Golgi apparatus membrane, Lipid-anchor
Target Information
GBP1 or guanlyate binding protein 1 is a 68 kDa GTPase and member of the GTPase1 family of molecules. GBP1 expression is induced by Interferon g as well as other inflammatory cytokines including IL-1a, IL-1b and TNF-a. Induction of GBP1 expression by poly I:C, a prototypical TLR3 ligand, as well as GBP1 expression dependency on NF-kB and IFNa response elements implicate TLR pathway involvement. Inversely proportional expression of GBP1 and MMP-1 as well as other related molecules to adhesion and migration show a clear functional role of GBP1 in suppressing angiogenesis and migration especially of endothelial cells. As a regulatory molecule it is centrally involved in signal transduction of inflammatory and cell migration networks.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GBP-1; GTP-binding protein 1; Guanine nucleotide-binding protein 1; guanylate binding protein 1, interferon-inducible, 67kDa; Guanylate-binding protein 1; huGBP-1; Interferon-induced guanylate-binding protein 1; unnamed protein product
Gene Aliases: GBP1; hGBP1
UniProt ID: (Human) P32455
Entrez Gene ID: (Human) 2633
nucleotide binding
lipopolysaccharide binding
actin binding
GTPase activity
obsolete small monomeric GTPase activity
guanosine-diphosphatase activity
protein binding
GTP binding
hydrolase activity
GDP binding
enzyme binding
cytokine binding
spectrin binding
identical protein binding
protein homodimerization activity
Hsp90 protein binding
immune system process
negative regulation of interleukin-2 production
defense response to bacterium
defense response to protozoan
innate immune response
regulation of calcium-mediated signaling
negative regulation of T cell receptor signaling pathway
protein maturation
defense response to virus
cytolysis in other organism
negative regulation of ERK1 and ERK2 cascade
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to tumor necrosis factor
protein localization to vacuole
positive regulation of pyroptotic inflammatory response
non-canonical inflammasome complex assembly
negative regulation of substrate adhesion-dependent cell spreading
regulation of protein localization to plasma membrane
negative regulation of protein localization to plasma membrane
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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