Proteintech

GCSH Polyclonal Antibody

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Antibody Testing Data (23)

GCSH Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 23

GCSH Antibody (16726-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using GCSH antibody 16726-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

GCSH Antibody in Immunohistochemistry (Paraffin) (IHC (P))

GCSH Antibody in Immunoprecipitation (IP)

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Product Details

16726-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:400

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

GCSH Fusion Protein Ag10174 (1-173 aa encoded by BC000790)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.36 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MALRVVRSV RALLCTLRAV PLPAAPCPPR PWQLGVGAVR TLRTGPALLS VRKFTEKHEW VTTENGIGTV GISNFAQEAL GDVVYCSLPE VGTKLNKQDE FGALESVKAA SELYSPLSGE VTEINEALAE NPGLVNKSCY EDGWLIKMTL SNPSELDELM SEEAYEKYIK SIEE (1-173 aa encoded by BC000790)

Target Information

The glycine cleavage system catalyzes the degradation of glycine. The H protein (GCSH) shuttles the methylamine group of glycine from the P protein (GLDC) to the T protein (GCST).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

FDX1 regulates cellular protein lipoylation through direct binding to LIAS.

bioRxiv : the preprint server for biology

Dreishpoon MB,Bick NR,Petrova B,Warui DM,Cameron A,Booker SJ,Kanarek N,Golub TR,Tsvetkov P

Sat Feb 04 00:00:00 EST 2023

Blocking glycine utilization inhibits multiple myeloma progression by disrupting glutathione balance.

Nature communications

Xia J,Zhang J,Wu X,Du W,Zhu Y,Liu X,Liu Z,Meng B,Guo J,Yang Q,Wang Y,Wang Q,Feng X,Xie G,Shen Y,He Y,Xiang J,Wu M,An G,Qiu L,Jia W,Zhou W

Mon Jul 11 00:00:00 EDT 2022

Proteomic analysis reveals the heterogeneity of metabolic reprogramming in lacrimal gland tumors.

Experimental eye research

Yang J,Li Y,Gao W,Feng Y,He X,Ni H,Song X,Fan J

Wed Jun 01 00:00:00 EDT 2022

Proteomic analysis reveals the heterogeneity of metabolic reprogramming in lacrimal gland tumors.

Experimental eye research

Yang J,Li Y,Gao W,Feng Y,He X,Ni H,Song X,Fan J

Wed Jun 01 00:00:00 EDT 2022

Copper induces cell death by targeting lipoylated TCA cycle proteins.

Science (New York, N.Y.)

Tsvetkov P,Coy S,Petrova B,Dreishpoon M,Verma A,Abdusamad M,Rossen J,Joesch-Cohen L,Humeidi R,Spangler RD,Eaton JK,Frenkel E,Kocak M,Corsello SM,Lutsenko S,Kanarek N,Santagata S,Golub TR

Fri Mar 18 00:00:00 EDT 2022

Vincetoxicum arnottianum modulates motility features and metastatic marker expression in pediatric rhabdomyosarcoma by stabilizing the actin cytoskeleton.

BMC complementary medicine and therapies

Adamus A,Ali I,Vasileiadis V,Al-Hileh L,Lisec J,Frank M,Seitz G,Engel N

Tue May 04 00:00:00 EDT 2021

Obesity increases hepatic glycine dehydrogenase and aminomethyltransferase expression while dietary glycine supplementation reduces white adipose tissue in Zucker diabetic fatty rats.

Amino acids

Simmons RM,McKnight SM,Edwards AK,Wu G,Satterfield MC

Thu Oct 01 00:00:00 EDT 2020

Glycine cleavage system determines the fate of pluripotent stem cells via the regulation of senescence and epigenetic modifications.

Life science alliance

Tian S,Feng J,Cao Y,Shen S,Cai Y,Yang D,Yan R,Wang L,Zhang H,Zhong X,Gao P

Tue Oct 01 00:00:00 EDT 2019

Acute loss of iron-sulfur clusters results in metabolic reprogramming and generation of lipid droplets in mammalian cells.

The Journal of biological chemistry

Crooks DR,Maio N,Lane AN,Jarnik M,Higashi RM,Haller RG,Yang Y,Fan TW,Linehan WM,Rouault TA

Fri May 25 00:00:00 EDT 2018

Acute loss of iron-sulfur clusters results in metabolic reprogramming and generation of lipid droplets in mammalian cells.

The Journal of biological chemistry

Crooks DR,Maio N,Lane AN,Jarnik M,Higashi RM,Haller RG,Yang Y,Fan TW,Linehan WM,Rouault TA

Fri May 25 00:00:00 EDT 2018

GCSH antisense regulation determines breast cancer cells' viability.

Scientific reports