Invitrogen

GFAP Monoclonal Antibody (ASTRO6)

Product Image Gallery

GFAP Antibody — Published figure using GFAP monoclonal antibody (Product # MA5-12023)

GFAP Antibody in Immunohistochemistry (IHC) — Formalin-fixed, paraffin-embedded human brain stained with GFAP antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of astrocytes.

GFAP Antibody in Western Blot (WB) — Western blot of Glial Fibrillary Acidic Protein using Glial Fibrillary Acidic Protein Monoclonal Antibody (Product # MA5-12023) on IMR-5 Cells.

GFAP Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of GFAP (green) showing staining in the in the cytoplasm of U251 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a GFAP monoclonal antibody (Product # MA5-12023) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

GFAP Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of GFAP showing staining in the cytoplasm of paraffin-embedded human cerebellum tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a GFAP monoclonal antibody (Product # MA5-12023) diluted in 3% BSA-PBS at a dilution of 1:200 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

1:20-1:200

Immunofluorescence (IF)

1:20-1:200

Immunohistochemistry (Paraffin) (IHC (P))

1-2 µg/mL

Western Blot (WB)

1-2 µg/mL

Published Applications

Flow Cytometry (Flow)

See 1 publication below

Immunohistochemistry (Paraffin) (IHC (P))

See 4 publications below

Immunocytochemistry (ICC)

See 5 publications below

Immunohistochemistry (Frozen) (IHC (F))

See 3 publications below

Immunofluorescence (IF)

See 1 publication below

Immunohistochemistry (IHC)

See 21 publications below

Western Blot (WB)

See 1 publication below

Product Specifications

Species Reactivity

Chicken, Human, Pig, Rat

Published species

Human, Mouse, Pig, Rat

Host / Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

ASTRO6

Immunogen

Glial Fibrillary Acidic Protein

Conjugate

Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C

RRID

AB_10984338

Product Specific Information

MA5-12023 targets Glial Fibrillary Acidic Protein in IF, IHC (P), and WB applications and shows reactivity with Chicken, Human, Porcine, and Rat samples.

The MA5-12023 immunogen is glial Fibrillary Acidic Protein.

MA5-12023 was successfully used to detect astrocytes in E18 Sparague Dawley primary cortical cells.

Target Information

GFAP (Glial fibrillary acidic protein) is a member of the class III intermediate filament protein family. GFAP is heavily, and specifically, expressed in astrocytes and certain astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In addition, neural stem cells strongly express GFAP. Antibodies to GFAP are very useful as markers of astrocytic cells. In addition, many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. GFAP is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing of the GFAP gene results in multiple transcript variants encoding distinct isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.