|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A peptide corresponding to amino acids 377 to 391 of human GFR alpha 2|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||5 µg/ml|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
In Western blot applications, this antibody detects a band at ~52kDa. A suggested positive control is Hela cell lysate.
PA5-19874 can be used with blocking peptide PEP-0254.
Members of the glial cell line-derived neurotrophic factor (GDNF) family, including GDNF and neurturin (NTN) play key roles in the control of vertebrate neuron survival and differentiation. Physiological responses to NTN require the presence of a novel glycosylphosphadidylinositol linked protein NTNRalpha, which is a cell surface receptor for NTN. The cDNAs encoding NTNRalpha from human, rat, chicken, and mouse have been cloned recently. NTNRalpha was also termed GDNFR-b, Ret ligand 2 (RETL2) or TGFbeta-related neurotrophic factor receptor 2 (TrnR2) and nominated as GFR-a-2 recently. GFR-a-2 binds NTN and mediates activation of RET receptor tyrosine kinase by both NTN and GDNF. Thus, NTN, GFR-a-2, and the Ret PTK form a complex to transduce NTN signal and to mediate NTN function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.