|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide, KLH conjugated, corresponding to residues R(403) T R A K W T T P S R S A A K V L T S M(422) of human GHRH-R.|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:1,000|
|Western Blot (WB)||1:3,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
PA3-117 detects GHRH-R in human samples.
PA3-117 has been successfully used in Western blot, immunocytochemistry and immunohistochemistry procedures. By Western blot, this antibody detects an ~40 kDa protein representing GHRH-R in human samples. In immunohistochemistry, GHRH-R immunoreactivity is predominantly localized at the plasma membrane.
The PA3-117 immunizing peptide corresponds to amino acid residues 403-422 from human GHRH-R.
Growth Hormone Releasing Hormone (GHRH) is a member of a family of peptide hormones that also includes VIP (vasoactive intestinal polypeptide) and PACAP (pituitary adenylate cyclase-activating polypeptide). It is a hypothalamic peptide that binds to G-protein coupled receptors to stimulate secretion and synthesis of growth hormone (GH). GHRH receptor proteins have been identified on the plasma membrane of primary human tumor cells, and are thought to be involved in the growth of a variety of cancer cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Immunocytochemical localisation of plasma membrane GHRH receptors in human tumours using a novel anti-peptide antibody.
PA3-117 was used in immunocytochemistry, immunohistochemistry and western blot to study the GHRH receptor immunocytochemical localization by a novel antibody in human tumours
|Schulz S,Röcken C,Schulz S||European journal of cancer (Oxford, England : 1990) (42:2390)||2006|