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Invitrogen
GLAST Polyclonal Antibody
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FIGURE: 1 / 1
GLAST Antibody (PA5-90649) in WB
Western blot analysis of GLAST in extracts of various cell lines. Samples were incubated with GLAST Polyclonal antibody (Product # PA5-90649) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-90649
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 453-542 of human EAAT1/EAAT1/SLC1A3 (NP_0041633)
Conjugate
Form
Liquid
Concentration
0.87 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: IVLTSVGLPT DDITLIIAVD WFLDRLRTTT NVLGDSLGAG IVEHLSRHEL KNRDVEMGNS VIEENEMKKP YQLIAQDNET EKPIDSETKM; Positive Samples: 293T, U-251MG, HeLa, H460, C6, Rat lung; Cellular Location: Membrane, Multi-pass membrane protein
Target Information
This gene encodes a member of a member of a high affinity glutamate transporter family. Mutations in this gene are associated with episodic ataxia, Type 6. Alternative splicing results in multiple transcript variants.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: EAAT-1; Excitatory amino acid transporter 1; FLJ25094; GLAST; GLAST-1; Glial glutamate transporter; Glial high affinity glutamate transporter; GluT-1; glutamate transporter; glutamate/aspartate transporter; glutamate/aspartate transporter 1; High-affinity neuronal glutamate transporter; Sodium-dependent glutamate/aspartate transporter 1; solute carrier family 1 (glial high affinity glutamate transporter), member 3; Solute carrier family 1 member 3; solute carrier family 1, member 3; unnamed protein product
Gene Aliases: AI504299; B430115D02Rik; EA6; EAAT1; GLAST; GLAST-1; GLAST1; GLU-T; GluT-1; Gmt1; MGluT1; SLC1A3
UniProt ID: (Human) P43003, (Rat) P24942, (Mouse) P56564
Entrez Gene ID: (Human) 6507, (Rat) 29483, (Mouse) 20512
L-glutamate transmembrane transporter activity
high-affinity glutamate transmembrane transporter activity
protein binding
acidic amino acid transmembrane transporter activity
neutral amino acid transmembrane transporter activity
symporter activity
glutamate:sodium symporter activity
glutamate binding
amino acid binding
metal ion binding
sodium:dicarboxylate symporter activity
primary active transporter
neurotransmitter uptake
ion transport
neurotransmitter transport
amino acid transport
cellular sodium ion homeostasis
chemical synaptic transmission
sensory perception of sound
response to xenobiotic stimulus
response to light stimulus
gamma-aminobutyric acid biosynthetic process
response to wounding
neutral amino acid transport
L-glutamate transport
cranial nerve development
auditory behavior
response to antibiotic
cell morphogenesis involved in neuron differentiation
positive regulation of synaptic transmission
neuromuscular process controlling balance
L-glutamate import
transepithelial transport
D-aspartate import
cellular response to cocaine
potassium ion transmembrane transport
import into cell
L-glutamate import across plasma membrane
L-aspartate import across plasma membrane
transport across blood-brain barrier
chloride transmembrane transport
glutamate metabolic process
glutamate biosynthetic process
response to inorganic substance
sodium ion transmembrane transport
response to drug
malate-aspartate shuttle
L-glutamate transmembrane transport
positive regulation of defense response to virus by host
transport
dicarboxylic acid transport
response to amino acid
mitophagy in response to mitochondrial depolarization
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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