Immunofluoresce staining of GLI1 using GLI1 polyclonal antibody (Product # PA5-23411) at 10 ug/ml. Top panel: C666-1 malignant human nasopharyngel carcinoma cell line. Bottom panel: OKF6 tert-1 human oral epithelial cell line. GLI1 was more highly expressed in the C666-1 than in the OKF6 tert-1 cells.
|Tested species reactivity||Human, Non-human primate|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (954 PNLPNHKSGSYPTPSPC 970) of GLI1 protein.|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||10 µg/ml|
|Immunofluorescence (IF)||10 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||5 µg/ml|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
GLI1 is a protein originally isolated in human glioblastoma. The GLI proteins are the effectors of Sonic Hedgehog (Hh) signaling and have been shown to be involved in cell-fate determination, proliferation and patterning in many cell types and most organs during embryo development. GLI1 was originally isolated from a glioma tumour and has been found to be up regulated in many tumors including muscle, brain and skin tumors such as Basal cell carcinoma (BCC). Shh and the Gli genes are normally expressed in hair follicles, and skin tumours expressing Gli1 may arise from hair follicles. The level of Gli1 expression correlates with the tumor grade in bone and soft tissue sarcomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.