|Tested species reactivity||Human, Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to a region within amino acids 755 and 810 of mouse Gli1|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Contains||0.025% Proclin 300|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
PA5-32206 targets Gli1 in IHC (P) and WB applications and shows reactivity with Human and mouse samples.
The PA5-32206 immunogen is synthetic peptide corresponding to a region within amino acids 755 and 810 of Human Gli1.
May regulate the transcription of specific genes during normal development. May play a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling and thus cell proliferation and differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Genotype tunes pancreatic ductal adenocarcinoma tissue tension to induce matricellular fibrosis and tumor progression.
PA5-32206 was used in immunohistochemistry to propose that epithelial tension and matricellular fibrosis contribute to the aggressiveness of SMAD4 mutant pancreatic tumors
|Laklai H,Miroshnikova YA,Pickup MW,Collisson EA,Kim GE,Barrett AS,Hill RC,Lakins JN,Schlaepfer DD,Mouw JK,LeBleu VS,Roy N,Novitskiy SV,Johansen JS,Poli V,Kalluri R,Iacobuzio-Donahue CA,Wood LD,Hebrok M,Hansen K,Moses HL,Weaver VM||Nature medicine (22:497)||2016|