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Invitrogen
GLI1 Polyclonal Antibody
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FIGURE: 1 / 3
GLI1 Antibody (PA5-81184) in ICC/IF
Immunofluorescence staining of GLI1 in HeLa and HepG2 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with GLI1 Polyclonal Antibody (Product # PA5-81184, 1:1,000) at 4°C overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red). Positive staining was localized to cytoplasm and nucleus.
Product Details
PA5-81184
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to the N-terminus of the Human GLI1
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A, Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This product is preservative free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%-0.1%).
This antibody has specificity for Human GLI1.
Target Information
This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GLI-Kruppel family member GLI1; Glioma-associated oncogene; glioma-associated oncogene 1; glioma-associated oncogene homolog 1 (zinc finger protein); Oncogene GLI; Zinc finger protein GLI1
Gene Aliases: GLI; GLI1
UniProt ID: (Human) P08151
Entrez Gene ID: (Human) 2735
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
DNA binding
chromatin binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
protein binding
microtubule binding
transcription regulatory region DNA binding
metal ion binding
DNA-binding transcription factor
osteoblast differentiation
transcription, DNA-templated
smoothened signaling pathway
spermatogenesis
ventral midline development
positive regulation of cell proliferation
regulation of smoothened signaling pathway
response to wounding
epidermal cell differentiation
dorsal/ventral pattern formation
proximal/distal pattern formation
cerebellar cortex morphogenesis
smoothened signaling pathway involved in regulation of cerebellar granule cell precursor cell proliferation
pituitary gland development
lung development
regulation of osteoblast differentiation
positive regulation of DNA replication
positive regulation of smoothened signaling pathway
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
digestive tract morphogenesis
notochord regression
canonical Wnt signaling pathway
negative regulation of canonical Wnt signaling pathway
liver regeneration
regulation of hepatocyte proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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